Dip and Read™ Biosensors and Assays

Ready-to-Use

Octet^® biosensors and assay kits are configured in a standard 96-well format, for your convenience. They are compatible with all Octet instruments (whether using 96- or 384-well plates). Both kits and biosensors simplify quantitation and kinetics assays by eliminating throughput limitations caused by microfluidic flow, surface regeneration, and sample purification.

Biosensors

ForteBio biosensors are coated with a proprietary biocompatible matrix that is uniform and non-denaturing with minimal non-specific binding. The tips are derivatized and ready for use in a diverse set of biomolecular applications. Biosensors can also be customized for unique applications. Multiple layers can be bound and detected, opening a vast array of approaches to detecting and characterizing biological interactions. To date, over 2.5 million biosensor assays have been run on Octet systems, attesting to the high reproducibility, accuracy, precision and robustness of the platform. Octet biosensors are manufactured in a ISO 9001:2008 certified facility.

Each type of Octet Biosensor is designed to analyze specific biomolecular interactions.  A range of surface chemistries is available. Refer to the tables below to select the appropriate biosensor for your needs.

Biosensor Selection Guide

Biosensor Description Aminopropylsilane (APS) Adsorption of proteins and membrane fractions through hydrophobic moieties.  Kinetic applications include Adsorption of proteins and membrane fractions through hydrophobic moieties for kinetic screening and affinity characterization (ka, kd,KD). Learn more: download the Aminopropylsilane (APS) Biosensors datasheet Amine Reactive (AR) Carboxylate functionalized surface to allow covalent coupling of proteins via EDC/NHS mediated amide bond formation.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd,KD), and hit validation. Learn more: download the Amine Reactive (AR) Biosensors datasheet Amine Reactive Second-Generation (AR2G) Second-generation carboxylate functionalized surface allows covalent coupling of proteins via EDC/s-NHS mediated amide bond formation.  The second-generation surface provides increased loading density, more robust loading conditions and decreased non-specific binding compared to the original AR biosensor for most proteins. Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd,KD), and hit validation. Requires AR2G Assay Kit (part no. 18-5095). Learn more: download the Amine Reactive Second-Generation (AR2G) Biosensor Datasheet and Technical Note 26: Dip and Read Amine Reactive Second-Generation (AR2G) Biosensors Anti-GST (GST) High affinity anti-GST antibody enables direct and rapid quantitation of GST-tagged biomolecules and easy and reliable capture of GST-tagged biomolecules for rapid kinetic analysis. Learn more: download the [Unknown document (id=233)], Technical Note 29: GST Biosensor Kinetics Assays and Technical Note 30: GST Biosensor Quantitation Assays Anti-hIgG Fc Capture (AHC) Immobilization of human IgG or other human Fc-containing proteins by binding to the human Fc region.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd,KD), epitope binning, and hit validation. Learn more: download the Anti-human IgG Fc Capture (AHC) Biosensors datasheet Anti-mlgG Fc Capture (AMC) Binds the Fc portion of IgG1, IgG2a, IgG2b  for capture based immobilization.  Applications include kinetic analysis of antibody-antigen interactions (ka, kd, KD), and off-rate screening.  IgG3 should be evaluated on a case-by-case basis. Learn more: download the Anti-Mouse IgG Fc Capture (AMC) Biosensors datasheet Anti-human IgG Fc Binds specifically to the Fc portion of human IgGs and other proteins containing a human Fc region.  Applications include quantitation of human IgG and proteins containing the human Fc region in cell line development, clone selection, process optimization, and production monitoring. Learn more: download the Anti-Human IgG (Fc specific) Biosensors datasheet Anti-murine IgG Fv Binds specifically to the Fv(ab')2 portion of mouse and rat IgGs.  Applications include quantitaiton of mouse and rat IgG in cell line development, clone selection, process optimization, and production monitoring. Learn more: download the Anti-Murine IgG Fv Biosensors datasheet Anti-Penta-HIS (HIS) Uses the Qiagen Penta-HIS antibody to bind with high affinity HIS taged recombinant proteins. Learn more: download the Anti-Penta-HIS (HIS) Biosensors datasheet Protein A Binds with high affinity to the Fc region of human IgGs.  Binds with moderate affinity to many subtypes of mouse and rabbit IgG.  Applications include quantitation of IgG in cell line development, clone selection, process optimization, and production monitoring. Learn more: download the Protein A Biosensors datasheet Protein G Binds with high affinity to the Fc region of murine, rat, goat, and bovine IgGs.  Binds with moderate affinity to many subtypes of human IgG.  Applications include quantitation of IgG in cell line development, clone selection, process optimization, and production monitoring. Learn more: download the Protein G Biosensors datasheet Protein L Binds with high affinity to most mouse, rat and human immunoglobulins containing a kappa light chain. Does not bind goat, bovine, rabbit, sheep IgG. Applications include quantitation of FAb fragments and of IgG in serum based culture. Learn more: download the Protein L Biosensors datasheet Streptavidin (SA) Streptavidin-coated biosensor with high binding capacity.  Immobilizes biotinylated antibodies, proteins, and nucleic acids to form a stable surface.  Quantitation applications include antibody and protein quantitation in cell line development, clone selection, process optimization, and production monitoring.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd,KD), epitope binning, and hit validation. Learn more: download the Streptavidin (SA) Biosensors datasheet Super Streptavidin (SSA) Streptavidin-coated biosensor with a very high density of biotin binding sites.  Immobilizes biotinylated proteins, peptides, nucleic acids and small molecules to form a stable surface.  Kinetic applications include antibody, protein, peptide, and small molecule kinetic screening, affinity characterization (ka, kd,KD), epitope binning, and hit validation. Learn more: download the Super Streptavidin (SSA) Biosensors datasheet

Literature References

References to ForteBio's products in published literature.

Suggest a Biosensor

Ideas for new biosensors are always welcome. Use our biosensor suggestion form to send your recommendation for a new biosensor offering to the ForteBio product development team.

Don't see the biosensor chemistry you need? Visit our Custom Services web page to find out how ForteBio can make a biosensor just for you.

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