Dip and Read™ Protein L Biosensors
For Whole Molecule Antibody and Antibody Fragment Quantitation

Protein L biosensors provide a rapid and direct method for quantitating a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb fragments and single chain variable fragments, from buffer, conditioned media or complex matrices. The recombinant Protein L, which is factory-immobilized onto the biosensor, recognizes a wider range of antibody classes than both Protein A and Protein G (Table 1), including IgG, IgM, IgA, IgE and IgD. The Protein L biosensor is especially useful for quantifying antibodies and antibody fragments from serum based culture because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements. Note that Protein L does not recognize all kappa light chains.  For example, Protein L recognizes mouse VkI (but not VkII, VkIII and VkIV) and human VkI, VkIII and VkIV (but not VkII).

Learn more: download Protein L Biosensors datasheet

Key Features

• Broader recognition of Ig isotypes and species than Protein A or G
• Regenerable and cost-effective format
• Binds whole molecule Ab and FAb fragments
• Quantitate in crude matrices

For More Information

Visit our literature page to download technical notes, application notes, posters and customer presentations.

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