Featured Highights

BLItz performs label-free assays in a drop of sample. Determine protein presence, quantitate proteins, analyze binding kinetics and develop immunoassays with this compact personal assay system.

Glutathione-S-transferase (GST) is commonly fused to recombinant proteins as a means of facilitating detection, purification and increasing solubility. The anti-GST biosensor consists of a high affinity anti-GST antibody pre-immobilized on a ForteBio biosensor. In conjunction with the Octet System, the anti-GST biosensor provides a rapid and label-free method for GST-tagged protein quantitation and kinetic analysis. The high specificity of the antibody-based biosensor enables direct analysis of GST analytes in crude lysates, column eluents, cell lysates and cell culture supernatants, serving as a time-saving alternative to traditional analytical methods.

The AR2G biosensor is a newly engineered biocompatible surface matrix that delivers enhanced performance for kinetic assays compared to the original Amine Reactive (AR) biosensor for most proteins. AR2G routinely achieves greater signal intensity than the AR biosensor during both loading (immobilization of ligand) and association (detection of analyte) assay steps while delivering the same reliable kinetic values. A decrease in non-specific binding events to the AR2G surface compared to AR has also been demonstrated.

ForteBio's new Octet RED96 system is a multi-functional, label-free, real-time analysis instrument. It is ideal for rapidly measuring concentration of proteins and other biomolecules, measuring kinetics and affinity, and screening protein-protein and protein-small molecule interactions. The Octet RED96 can be used for a wide range of analyses including IgG and other protein titer, bioprocess development, quality analysis, crude antibody screening, epitope binning/mapping, ligand binding assays, small molecule and fragment screening and analysis, elucidating cell signaling mechanisms and infectious disease monitoring. Replace your stodgy HPLC, ELISA and other single-purpose microfluidics instruments for a unique, cutting-edge, protein analysis system that accelerates and simplifies your work.

Protein L biosensors provide a rapid, direct method for quantitating a broad set of kappa light chain-containing immunoglobulins, including whole molecules, FAb fragments and single chain variable fragments, from buffer, conditioned media or complex matrices. The recombinant Protein L, factory-immobilized onto the biosensor, recognizes a wider range of antibody classes than both Protein A and G, including IgG, IgM, IgA, IgE and IgD. The Protein L biosensor is especially useful for quantifying antibodies and antibody fragments from serum based culture because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements.

ForteBio’s new Octet RED384 and Octet QK384 systems are designed for high-throughput, label-free biomolecular quantitation and kinetic characterization, with a large dynamic range for protein quantitation. Get accurate concentration, kinetic constants, and affinity data for protein-protein, small molecule-protein and other fast-binding interactions — all with Dip and Read simplicity. The Octet RED384 and QK384 systems are easy to set up and when used in conjunction with robotics, provide complete unattended operation for your assays. An advanced quantitation module enhances quantitation sensitivity to sub-ng/mL. With the lowest cost-per-data point, label-free quantitation and kinetics are available to more laboratories than ever before.

The a href="octet.html">Octet QKe system is the newest addition to the Octet family of real-time, label-free biomolecular interaction analysis instruments. It is an enhanced performance version of the first-generation Octet QK instrument. Two acquisition rate settings and a higher-performance spectrometer combine to provide greater sensitivity and wider dynamic range for quantitation and kinetic analysis. In addition, biosensor re-racking provides enhanced flexibility and operational cost savings for running assays.

The Dip and Read Residual Protein A Detection Kit contains biosensors and all the reagents required to quantitate Protein A, or biosimilars such as MabSelect SuRe, in column fractions. The Dip and Read Residual Protein A Detection Kit provides the sensitivity and accuracy needed for this critical test along with minimal hands-on time and an easy to use protocol.

The Dip and Read Immunogenicity Assay Kit offers the needed sensitivity, required drug tolerance, and flexibility to detect low affinity ADA by providing multiple protocols requiring no plate-washing steps.

The six-histidine or penta-histidine peptide tag (HIS-tag) is a common peptide tag fused to recombinant proteins during cloning. Many tools have been developed enabling the tag to be used for easy detection and purification of tagged proteins. The Anti-Penta-HIS biosensor, for use on the Octet system, now provides a rapid, label-free method for quantification of HIS-tagged proteins. This biosensor comes with the highly specific Qiagen Penta-HIS antibody pre-immobilized on the surface and is ready to use for specific detection and quantitation of HIS-tagged proteins.

• Introduction to the new Octet RED System: extended detection capabilities for both quantitation and kinetics
• Reproducible quantitative kinetic analysis of small molecules in comparison to published data
• Protein:small molecule blind study results with one of ForteBio's collaborators
• Question and answer session with one of ForteBio's Octet RED System specialists

The Anti-Human IgG Fc Capture (AHC) biosensor is designed for kinetic characterization of macromolecular interactions between proteins containing an Fc region and a target analyte. This biosensor enables the immobilization of human IgG or other protein containing a human Fc region using a high-affinity anti-human Fc-specific antibody. The biosensor surface has been designed to provide stable interactions and can be regenerated, making it particularly suitable for high-throughput kinetics applications. AHC biosensors can be used with many sample types, including purified proteins and cell culture supernatants.

ForteBio's Super Streptavidin Biosensors, in conjunction with the Octet RED System, are designed for kinetic analysis of small protein and/or small molecule interactions.

The Octet Installation Qualification (IQ) and Operational Qualification (OQ) manual provides the procedures to document and verify that the correct Octet System was ordered, delivered and installed according to ForteBio specifications. ForteBio offers Octet customers the documentation and services needed to assure successful validation of the Octet System. The manual can be ordered as a package with the Compliance Validation Software Module or can be ordered separately.