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-All Categories- Customer Posters/ PresentationsPosters and Presentations by ForteBioTechnical NotesProduct Package InsertsSoftware Product InformationCurrent Product Datasheets
-All Applications- Kinetic ScreeningAntibody QuantitationKinetic CharacterizationBindingCustom quantitationAmine Reactive Biosensors: Quantitation
-All Products- 384-Well Tilted-Bottom Microplate (Case)384-Well Tilted-Bottom Microplate (Pack)Amine Coupling Reagent KitAmine Reactive (AR), CaseAmine Reactive (AR), PackAmine Reactive (AR), TrayAminopropylsilane (APS), CaseAminopropylsilane (APS), PackAminopropylsilane (APS), TrayAnti-hIgG Fc Capture (AHC), CaseAnti-hIgG Fc Capture (AHC), PackAnti-hIgG Fc Capture (AHC), TrayAnti-Human IgG Fc, CaseAnti-Human IgG Fc, PackAnti-human IgG Fc, TrayAnti-Murine IgG Fv, CaseAnti-Murine IgG Fv, PackAnti-Murine IgG Fv, TrayAnti-Penta-HIS (HIS) Biosensors, CaseAnti-Penta-HIS (HIS) Biosensors, PackAnti-Penta-HIS (HIS) Biosensors, TrayBiosensor DispenserCFR Part 11 Software for Octet SystemsDip and Read Immunogenicity Assay KitDip and Read Residual Protein A Detection Kit IgG Calibrators, Extended RangeIgG Calibrators, Standard RangeImmunogenicity ReagentInstallation and Operational Qualification Manual (IQOQ)IQOQ Validation ServiceKinetics Buffer 10XMES Buffer, pH 4.0MES Buffer, pH 5.0MES Buffer, pH 6.0Octet Q SystemOctet Q to QK UpgradeOctet QK Software UpgradeOctet QK SystemOctet QK384 SystemOctet QKe SystemOctet RED SystemOctet RED384 SystemPerformance Validation ServiceProtein A, CaseProtein A, PackProtein A, TrayProtein G, CaseProtein G, PackProtein G, TraySample DiluentSample Diluent with ProClin 300Sidekick StationSingle Seat License for Octet Data Analysis Software Current CFR Part 11 VersionSingle Seat License for Octet Data Analysis Software Current VersionSite License CFR Part 11 Data Analysis Software Version 6.xSite License Octet Data Analysis Software Version 6.xStreptavidin (SA), CaseStreptavidin (SA), PackStreptavidin (SA), TraySuper Streptavidin (SSA), CaseSuper Streptavidin (SSA), PackSuper Streptavidin (SSA), Tray
Current Product Datasheets
ForteBio offers a range of high-quality, custom assay development services to help customers develop specific assays for its Octet platform of instruments, including host cell protein (HCP) detection assays. ForteBio’s HCP assays allow Octet users to cost-effectively accelerate their research efforts and maximize the utility of their Octet system. Since no two HCP detection assays are identical, the custom services team will work closely with each customer to tailor each assay to the customer’s specifications using customer-defined capture reagents and analytes. All custom products are developed by ForteBio’s world-class product development group to deliver the best quality-controlled products possible for the Octet platform. Custom HCP detection kits contain biosensors and all the reagents required to quantitate customer-designated host cell proteins.
ForteBio offers a range of high-quality, contract services to help our customers develop assays for its Octet platform of instruments. ForteBio’s services allow Octet users to cost effectively accelerate their research efforts and maximize the utility of their Octet system. These services have been designed to support the unique needs of clinical biotherapeutics development and enable ForteBio customers to obtain finished, qualitycontrolled biosensors and assay kits that are developed with the customers’ capture reagents and analytes.
ForteBio Streptavidin (SA) biosensors are designed for immobilization of biotin labeled proteins for use in assaying protein:protein interactions using the Octet system. The Octet system supports applications for kinetics characterization and quantitation of analytes binding to the immobilized protein.
The Octet QKe instrument is a new addition to the Octet family of real-time label-free biomolecular interaction analysis instruments. The Octet QKe has been designed to be an enhanced performance version of the Octet QK instrument. Three acquisition speed settings to customize assay performance and a higher performance spectrometer combine to provide greater sensitivity and greater dynamic range for quantitation and kinetic analysis. In addition, biosensor re-racking ability provides enhanced flexibility and operational cost savings for running assays.
The TW384 microplate is a black, polypropylene, 384-well tiltedbottom plate designed for ForteBio's Octet family of real-time biomolecular interaction analysis instruments. It can be used on the Octet RED384 and Octet QK384 instruments, both of which accommodate the 384-microwell plate format. The TW384 microplate enables use of samples as small as 40 uL in Octet assays and reduces variability in the background signal, thus improving assay sensitivity, particularly beneficial for peptide, small molecule and fragment analysis.
ForteBio's Amine Reactive (AR) biosensors are a flexible tool for large molecule kinetic analysis and kinetic screening of large proteins with a primary amine. These AR biosensors enable coupling proteins to carboxylate groups on the biosensor surface via accessible amine groups within the target protein. Amine coupling can be fully automated online or can be batch immobilized offline. Additionally, regeneration conditions can be developed to enable reuse of the target protein on the biosensor for additional kinetic analyses.
The six-histidine or penta-histidine peptide tag (HIS-tag) is fused to recombinant proteins during cloning, and many tools enable using it for easy detection and purification of tagged proteins. The Anti-Penta-HIS biosensor provides a rapid, label-free method for quantification of HIS-tagged proteins using the Octet System. This biosensor comes with the highly specific Qiagen Penta-HIS antibody pre-immobilized on the surface and is ready to use for specific detection and quantitation of HIS-tagged proteins.
Overview of ForteBio's Super Streptavidin biosensors.
The Sidekick station is an accessory to ForteBio's Octet family of real-time label-free biomolecular interaction analysis instruments. Sidekick enables simultaneous and uniform loading of reagents onto all 96 biosensors in a biosensor tray. Target analyte and other reagents that do not require online signal monitoring can be loaded onto biosensors on the Sidekick, freeing the Octet instrument for other users. The Sidekick can be installed beside an Octet system and used in collaboration with it, or it can be used independently.
This data sheet describes the use and performance of the Residual Protein A Detection Kit in quantitating Protein A and biosimlars such as MabSelect SuRe.
The datasheet provides technical information on the capabilities and performance of the Octet RED384 and the Octet QK384 instruments.
Learn more about the new Dip and Read Immunogenicity Assay Kit for detecting anti-drug antibodies in human and animal samples.
The Octet family brochure provides an overview of Fortebio's solution for label-free, real-time analysis of biomolecules from small molecule fragments to large IgM antibodies. The benefits of using the platform are discussed along with some major applications. A detailed comparison of the capabilities of the Octet Q, Octet QK and the Octet RED instruments is also described.
The Anti-Human IgG Fc Capture (AHC) biosensor is designed for kinetic characterization of macromolecular interactions between proteins containing an Fc region and a target analyte. This biosensor enables the immobilization of human IgG or other protein containing a human Fc region using a high-affinity anti-human Fc-specific antibody. The biosensor surface has been designed to provide stable interactions and can be regenerated, making it particularly suitable for high-throughput kinetics applications. AHC biosensors can be used with many sample types, including purified proteins and cell culture supernatants.
Aminopropylsilane (APS) Biosensors are designed for hydrophobic or electrostatic immobilization of most proteins used in assaying protein:protein interactions.
ForteBio's Anti-Murine IgG Biosensors, in conjunction with the Octet System, are designed for monitoring antibody concentrations in mouse or rat sera and cell culture supernatants. Using Anti-Murine IgG Biosensors, the Octet System supports applications from assay development, clonal selection, and cell culture screening to concentration monitoring during development and manufacturing.
ForteBio's Protein A Biosensors, in conjunction with the Octet System, are designed for monitoring antibody concentrations from crude lysates and cell culture supernatants. Using Protein A Biosensors, the Octet System supports applications from cell culture screening to purification monitoring during the process development and production of therapeutics.
ForteBio's Anti-Human IgG Biosensors, in conjunction with the Octet System, are designed for monitoring antibody concentrations from crude lysates and cell culture supernatants. Using Anti-Human IgG Biosensors, the Octet System supports applications from cell culture screening to purification monitoring during the process development and production of therapeutics.
Customer Posters/ Presentations
A tutorial on the use of Octet systems for protein titer determination in bioprocessing applications. The role of Octet QK systems in the protein expression workflow at Pfenex is described in this June 15, 2010 issue of GEN magazine.
The Pseudomonas-based Pfēnex Expression TechnologyTM has proven to be a robust and cost effective platform for the production of numerous classes of therapeutic proteins, including various types of antibody derivatives. High cell densities in the fermentor along with high specific protein yields result in high target protein titers. Very high cell densities are also achievable even in small scale growth (96-well plates). An extensive tool box of unique host strains has also been developed. These strains have phenotypes selected to impact the amount of target protein produced and its solubility and activity. Coupled to an automated sample preparation platform, parallel through-put biolayer interferometry (BLI) allows hundreds of different expression strategies and host cell combinations to be rapidly tested for both quantity and functionality.
Presented by Arnout Gerritsen, Genmab at Screening Europe, February 2010. Bio-Layer Interferometry (BLI) plays an important role in the antibody discovery and development processes at Genmab. Integrating the quantitation, kinetic and affinity output of the Octet RED384 and Octet QK systems with ActivityBase enables Genmab to perform "high value" screening within early antibody discovery.
Dr. John Wang's presentation at SBS 2010 and CHI's DDC 2010 on the use of the Octet RED384 system for higher throughput screening of small molecule libraries and fragment libraries. Validation data from Roche is included along with results from an internal Maybridge library screen.
Dr. Yasmina Abdiche, Sr. Principal Scientist at Rinat-Pfizer discussed the versatility of the Octet QK384 system in screening crude antibodies and in epitope binning. She described the Octet systems as multiplexed biosensors that move sensors to samples and open up many possibilities for assay formats. The Octet system allowed batch immobilization of antibody from crude media onto 96 biosensors at-once offline; after screening her antigen samples in solution, she could batch regenerate all 96 biosensors offline to screen 150 sups in just 2 hours. The Octet quickly discriminated between tight, medium and weak binders and picked up some weak binders missed by ELISA. For epitope binning, the Octet QK384 was able to analyze 52 antibody pairs in just 2 hours including time for online coupling of 4 antibodies on 16 biosensors. In comparison, a Biacore 2000 only analyzed 39 antibody pairs in 6 hours even when excluding time for coupling 3 antibodies to biosensors and instrument prep time.
Dr Shirley Li presented this poster at SBS 2009 in Lille, France describing the first use of the ForteBio Octet Red and its label-free biolayer interferometry technology for successfully completing a fragment library screening campaign for a protein target. The unique features of the system allowed for rapid screening against immobilized target, minimal protein degradation, and the sensitivity to detect the binding of small molecules in the 200 MW range.
Dr. Yasmina Abdiche of Rinat-Pfizer talked about her experiences using the Octet QK and Octet RED systems to explore blocking assays for biotherapeutic product development.
She summarized that the Octet system is a reliable and versatile biosensor that is simple to use and is especially well-suited to blocking assays that are valuable to drug discovery. She said that Epitope binnings from Octet matched those from SPR and that solution affinities from Octet also matched those from SPR.
Dr. Brian Miller, Senior Scientist for Antibody Engineering at Biogen Idec presented a seminar at PEGS in Boston in April 2009 on the use of the Octet system at Biogen for Protein Quantitation, Off-rate screening of antibodies in crude samples, monoclonal antibody cross-blocking, epitope scanning and affinity ranking of purified antibodies.
Presentation from Jane McIninch Ph.D. at IBC Antibody Production Confernce March 2009. Content includes a comparison of the Octet to ELISA and HPLC showing good correlation with both assay types, but allowing a significant throughput improvement IgG titer determination.
This presentation, titled "The Versatile Role of Bio-layer Interferometry in Therapeutic Antibody Discovery and Development" was made by Dr. Arnout Gerritsen of Genmab at the IBC's Antibody Engineering and Therapeutics on December 9, 2008. In the presentation, Dr. Gerritsen discusses the use of Octet in Genmab processes for Protein Quantitation, Avidity ranking, Ligand blocking, IgG ranking and binding affinity determination.
This presentation was made by Dr. Frank Podlaski of Roche, Nutley, NJ at the Society for Biomolecular Screening (SBS) conference in Dresden, Germany in June 2008.
Dr. Podlaski talks about the extensive testing his laboratory did to evalue the use of the Octet® RED instrument for small molecule kinetic analysis. He finds that the Octet® RED affords many specific advantages including ease-of-use, fast assay development, ability to identify aggregating and reactive compounds, and the ability to assay complex or insoluble analytes.
Presented at AAPS Biotechnology in June '08
Kinetic analysis of RH sample in serum using Octet QK, ProteOn and T100
Author: Flora Berisha
Improving the Accuracy, Speed, and Reproducibility of Titer Determination for the Clone Selection Process
By: Keith A. Davis, Ph.D., Scientist WPGRD, Global Biologics, Pfizer Inc
Accurate titer determination remains a challenge, for Cell Line Development, with less than desirable precision, accuracy, and throughput. A new technology was evaluated that provides a > 10x decrease in hands-on time and turnaround time vs. both HPLC and ELISA while providing accuracy and precision comparable to that of Protein A HPLC without the HPLC waste or maintenance.
Introducing the Octet as a reliable novel biosensor in characterizing protein/protein interactions
The presentation describes the study of protein/protein interactions using the Octet, a novel parallel-processing label-free biosensor. Two applications are described, namely “1-shot kinetics” and blocking, and examples are provided to demonstrate that the technology is reliable when compared with Biacore. The Octet is a simple dip-and-read assay that harnesses bio-layer interferometry to detect molecular level interactions in real time through the use of disposable fiber-optic biosensors. The Octet is emerging as an attractive low-cost complement to Biacore.
Technology Workshop presented at BioProcess International in Boston, MA
Title: Use of ForteBIO Octet for Quantitative Titer Analysis in Biopharmaceutical Development
Presentation given by León García-Martínez, Ph.D., Associate Director of Antibody Therapeutics, Alder Biopharmaceuticals
Presented at Screening Europe, Barcelona, Spain in February 07
Presentation given by Pete Flynn, Director of Biochemistry Research, Kalobios Pharmaceuticals
Presented at 17th Annual Antibody Engineering Conference in San Diego, CA in December ‘06
Presented by Dr. Laura Lerner at Bioprocess International '06, San Francisco, CA
Poster presentation by Raven Biotechnologies @ WilBio Waterside conference in Chicago, IL - 2006
Posters and Presentations by ForteBio
ForteBio’s Octet Dip and Read™ assay platform provides fast, accurate and easy-to-use assay solutions for many bioproduction applications. Direct binding assays that detect proteins without the need for secondary reagents are complemented by multi-step assay methods, affording a large dynamic range from picograms/mL to milligrams/mL. Octet systems are currently utilized in various stages of biopharmaceutical manufacturing to replace HPLC and ELISA methods which suffer from long analysis times, lack of specificity, labor-intensive protocols and imprecision. This talk will highlight Octet assays to detect residual residual host cell protein contamination in CHO cells and residual protein A contamination in antibody preparations.
The poster was presented by ForteBio at the Peptalk conference in San Diego, January 11 to 15, 2010. The poster describes the use of a specific anti-penta HIS antibody pre-immobilized on an Octet biosensor to capture and quantify HIS-tag proteins in a label-free method that does not require any secondary detection antibody. Using this method, we tested proteins with molecular weights ranging from 13 kDa up to 43 kDa and determined the assay dynamic range to be about 0.25 to 200 micrograms per milliliter in both purified form and spiked into spent CHO cell media. This assay provides a very rapid and easy method for quantitation of HIS-tag proteins in both purified and crude media.
The Octet RED384 instrument uses biolayer interferometry (BLI) to measure molecular interactions and is well‐suited for label‐free screening of small molecule fragments. It is a 16‐channel robot friendly instrument that is compatible with 384‐and 96‐well plates, is capable of screening thousands of compounds per day by measuring the interaction of a compound with protein target that is attached to the tips of biosensors. This instrument measures interactions directly in a microtiterplate without the use of microfluidics, and generates binding profiles, responses, and kinetic constants that correlate with SPR including kon , koff , and KD.
Presented by John Proctor at BPI 2009 in Raleigh, NC Detection of residual Protein A in product purified using a Protein A, or similar column matrix such as MabSelect SuRe™, is a critical quality control step in antibody therapeutic development and production. During the IgG purification process, Protein A can leach from the purification column and co-elute with the IgG. This contaminant in the therapeutic mixture can cause adverse reactions in patients and thus must be detected and minimized. The Octet family of instruments utilizes a unique label-free “dip and read” detection technology to quantify biomolecules in solution. ForteBio has developed a new kit for use on this system to detect and quantify residual Protein A. The new Dip and Read™ Residual Protein A Detection Kit contains biosensors and all the reagents required to quantitate Protein A and MabSelect SuRe. The data presented here demonstrates that the Octet system can accurately and precisely detect 100 pg/mL Protein A or MabSelect SuRe in bioprocess samples.
John Proctor (Product Manager) introduced the new Immunogenicity Assay Kit at AAPS in Seattle June 2009. His presentation details the sensitivity and drug tolerance this new kit delivers in two fast and flexible formats.
We report a label-free method for determining kinetic constants for the binding of small molecule inhibitors (<500 Daltons) to carbonic anhydrase, including rate constants and affinity constants. These parameters are derived from data obtained using FortéBio’s Octet RED System, a label-free, 8-channel system that is compatible with 96-well microplates.
The Octet RED has increased sensitivity due to a 10X decrease in system noise. In addition the RED has a significant increase in data sampling rate, allowing for true parallel analysis of up to 8 interactions with a 96 well plate walk away capability. To demonstrate the performance of the instrument kinetic analysis of proteinprotein and protein-small molecules will be described.
Presented at Society of Biomolecular Screening in Seattle, WA, 2006
Presented at the Society of Biomolecular Screening in Seattle, WA, 2006
Quantitation of antibodies using a Protein A biosensor on the Octet
ForteBio's Octet - A Novel System for Measuring Protein Interactions in Antibody Development and Production
Efficient and cost-effective workflow for antibody bioprocessing
Development of the ForteBio® Octet System
Product Package Inserts
Product insert describing usage conidtions of the streptavidin biosensor.
Product insert describing usage conditions for the Super Streptavidin biosensors.
Product insert describing useage conditions for the Anti-Human IgG Fc Capture Biosensors.
Octet Biosensors for Label-Free Detection of protein binding Product Code: 18-5010, 18-5012, 18-5013
Octet Biosensors for Label-Free Detection of protein binding Product Codes: 18-5011, 18-5014, 18-5015
Octet Biosensors for Label-Free Detection of protein binding Product Code: 18-5022, 18-5023, 18-5024
Reagent Kit for use with Amine Reactive Biosensors Product Code: 18-5017
Software Product Information
The document lists major features of the Octet software in tabular format and compares versions 4.0, 6.1, 6.2 and 6.3.
Octet Systems RED, QKe, QK, RED384 and QK384 software version 6.3 User Guide for Data Analysis
For Octet RED, QKe and QK Systems, user guide for data acquisition for software version 6.3
Octet 384 systems (RED384 and QK384) data acquisition user guide for software version 6.3
This manual explains how to install the Sidekick Station, operate the Sidekick Station, set up and run batch loading and offline loading in advanced quantitation experiments, maintain the Sidekick and integrate the Sidekick to an automated laboratory system.
Octet RED User Guide for users running v6.0 Software
Update to 5.0 software User Guide
Octet RED User Guide v5.0
Key Features: Global Fitting Regeneration Serial sample dilutions in quantitation mode
Octet System Users Guide, Rev. D
Technical Notes
Protein G biosensors provide a rapid and direct method for quantification of many types of mammalian IgG from buffer, media or other complex matrices. Protein G, which is pre-immobilized onto the biosensor, binds to rodent species IgG and many other mammalian IgG with higher affinity than Protein A but does not bind IgM, IgD or IgA. Specificity for IgG makes Protein G biosensors useful for quantifying many species of IgG, including murine, goat, and bovine in the presence of other types of immunoglobulins or protein contaminants.
This technical note describes a rapid assay method for quantitation and subtype identification of human and mouse IgG. The antibodies can be quantified in buffer, serum-free media or lysates, using the ForteBio® Anti-Human IgG Fc or Anti-Murine Fv biosensors. Using the same biosensors, the subtype of the antibody can be identified using subtype-specific secondary antibodies. The flexible microplate-based format of the Octet platform allows up to 48 samples to be analyzed on an Octet QK or Octet RED system and up to 96 samples on an Octet QK384 or Octet RED384 system in 1 hour.
The six-histidine or penta-histidine peptide tag (HIS-tag) is a common peptide tag fused to recombinant proteins during cloning. Many tools have been developed enabling the tag to be used for easy detection and purification of tagged proteins. The Anti-Penta-HIS biosensor, for use on the Octet system, now provides a rapid, label-free method for quantification of HIS-tagged proteins. This biosensor comes with the highly specific Qiagen Penta-HIS antibody pre-immobilized on the surface and is ready to use for specific detection and quantitation of HIS-tagged proteins.
This technical note describes the use, including detailed protocols, and performance of the Residual Protein A Detection Kit in quantitating Protein A and biosimlars such as MabSelect SuRe.
Small molecule kinetics can be measured on the Octet RED and Octet RED384 instruments. This technical note provides guidelines for developing small molecule kinetic characterization methods using SSA biosensors. It includes procedures for: • Immobilizing biotinylated target protein onto SSA biosensors • Performing small molecule analysis experiments using the Octet RED384 and Octet RED systems • Analyzing and interpreting the small molecule binding data, including differentiating meaningful data from artifacts
Tech Note 17: Dip and Read Immunogenicity Assay Kit. Describes the protocols available for the Dip and Read Immunogenicity Assay Kit: an enzyme-linked bridging assay and a direct binding assay. These protocols work across the many drug types in the market today such as antibodies, proteins, and peptides and can be used with both human and animal samples. In conjunction with ForteBio's Octet instruments, the kit offers the needed sensitivity, the required drug tolerance, and the flexibility to detect both high and low affinity ADA by providing multiple protocols without any plate washing steps.
Tech Note 14: Regeneration Strategies for Streptavidin Biosensors on the Octet Platform In some applications, particularly kinetic screening, it may be advantageous to assay several protein samples using the same ligand-coated biosensor. To accomplish this, the target protein must be dissociated from the ligand-coated biosensor, regenerating the biosensor so that it can be used in another assay. This technical note provides guidelines to develop a successful regeneration protocol.
Tech Note 15: High Sensitivity Detection of Human IgG Using Protein A Biosensors. This technical note describes a new, rapid assay with a wide dynamic range for detecting low concentrations of human IgG using the Protein A biosensors in buffer, serum-free media and lysates. By taking advantage of the flexible 96-well based format of the Octet platform, one plate can be analyzed in less than one hour. In addition, the Protein A biosensors can be regenerated to provide a cost-effective and reproducible assay.
Tech Note 13: Development of a Quantitation Assay for His-Tagged Proteins. A label-free quantitation assay for 6XHis-tagged proteins can be developed using the Octet QK and Octet RED instruments and streptavidin biosensors. By taking advantage of the flexible design of the biosensor tray, nickel-activated Biotin-NTA or an anti- His-tag directed antibody can be batch-immobilized on streptavidin biosensors outside of the Octet QK or Octet RED instruments. These ligand-coated biosensors can be used to rapidly quantitate His-tagged protein(s).
Tech Note 10: Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors. This technical note outlines a general batch mode procedure for immobilizing a biotinylated ligand onto Streptavidin biosensors in the biosensor tray assembly on the benchtop. The goal is to develop a procedure that creates a binding surface having a maximum and reproducible response. The protocol outlines determining the optimal concentration and time for immobilization on-line and then the transfer of these parameters to off-line immobilization.
Tech Note 11: Biotinylating Antibody in Stocks Containing Carrier Protein. This technical note provides a detailed protocol for isolating antibodies from carrier protein and subsequently biotinylating the purified antibodies.
Tech Note 12: Biotinylating Very Small Quantities of Protein for Immobilization onto Streptavidin Biosensors. This technical note provides guidelines for biotinylating a protein of interest for use with Streptavidin biosensors on the Octet QK or Octet RED System, when <150 µg total protein is available.
Tech Note 9: Developing a Protein-Specific Quantitation Assay Using Streptavidin Biosensors. Guidelines for developing a custom quantitation assay using Streptavidin biosensors on Octet Systems.
Tech Note 8: Regeneration Strategies for Amine Reactive Biosensors on the Octet System. Provides examples for determining and validating an appropriate regeneration protocol.
Tech Note 7: Batch Immobilization of Protein Onto Amine Reactive Biosensors. After you've completed assay development, batch immobilization is ideal for producing batches of biosensors for your project. You have a choice of storage conditions - dry or wet.
Tech Note 5: Assay Development Module for Quantitation Assays. The new Quantitation Assay Development Module enables quantitation assays to be modified for specific needs. By changing the assay time (time interval at which data is taken for each set of 1-8 sensors) and/or the flow rate, the user can adjust the functional range of the assay. In the example shown below the anti-hIgG Fc biosensor assay has been changed from a functional range of 1-100 µg/mL to a new functional range of 0.2-25 µg/mL.
Tech Note 4: Dynamic Range Determination of an Antibody Using Protein A Biosensors. The Protein A assay for determining protein concentration measures the rate at which a protein of interest binds to the sensor surface. Different protein concentrations result in different binding rates. Using Protein A Biosensors on the Octet System, a dynamic range of 2-3 logs is typically achieved. The actual dynamic range will be dependent on the protein of interest.
Tech Note 6: Biotinylation of Protein for Immobilization Onto Streptavidin Biosensors. Protocol for biotinylation of proteins for use with Streptavidin biosensors.
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