Applications Overview

Understanding biomolecular interactions is at the core of therapeutic and drug development and production. Label-free, real-time methods are increasingly preferred, as they avoid artifacts caused by label interference. ForteBio’s Octet family of instruments provides label-free, real-time measurements in the analysis of protein:protein, protein:peptide, and protein:small molecule interactions. With unparalleled ease of use, the Octet family of instruments moves your workflow beyond the limitations of existing label-free methods. You get comprehensive characterization across a broad range of applications that are critical for efficient development and production.

Kinetic Characterization
The kinetic analysis of antibodies is critical to both the screening and selection of clones for development and thorough characterization throughout therapeutic development and production. With a dip and read label-free technique, ForteBio’s BLI-based systems are easy to learn. They also enable expanded kinetics analyses in a workflow that provides higher throughput without the limitations of flow-cell based systems.

Protein Quantitation in Bioprocessing
Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA.

Small Molecule and Small Peptides
Small molecule and peptide interactions that are extremely challenging to characterize are becoming an important focus in drug discovery. The Octet RED System’s high sensitivity and rapid data acquisition capabilities enable the analysis of low molecular weight molecules that interact extremely quickly — in a flexible platform that is easy to learn and more affordable in comparison to existing systems with similar capabilities.

Qualitative Kinetics Assays
Qualitative, real-time assays provide invaluable insight into biomolecular interactions that endpoint assays such as ELISA, cannot. With real-time binding sensorgrams and eight-channel parallel analysis, the Octet family of instruments provides a qualitative tool for examining epitope binning, antibody isotyping, and screening of expression clones.

Assay Development
Performing efficient assay optimization before proceeding to large-scale implementation saves time, materials, and money. Parallel eight-channel analysis without microfluidics allows a multitude of conditions to be assayed in parallel with minimal sample purification and preparation.