Applications Overview

 

ForteBio's Octet® and Pioneer family of instruments provides real-time measurements in the analysis of therapeutic biological molecules at different stages of development. The applications include but are not limited to protein:protein, protein:nucleic acids, protein:small molecules interactions and the analysis of large molecules such as virus-like particles. With unparalleled ease of use, the Octet and Pioneer family of instruments moves your workflow beyond the limitations of existing label-free methods. You get comprehensive characterization across a broad range of applications that are critical for efficient development and production.

Kinetic Apps Octet Instruments

Figure 1: Pall ForteBio offers solutions with wide fit within the discovery and development process.

In order to meet your high throughput demands for drug discovery, the Octet platform has eliminated the need for microfluidics and designed a simple Dip and Read method that allows simultaneous monitoring of large numbers of interactions in an automated manner. With the Octet systems, you can survey a number of different interactions rapidly, obtain accurate kinetic and affinity information and determine concentrations of your protein of interest in complex sample matrices. Ranging from the two-channel Octet K2 instrument to the un-paralleled 96-channel Octet HTX instruments, the Octet platform addresses a broad range of applications in various stages of the drug discovery and development process. With a wide range of ligand capture surfaces that allow for the immobilization of antibodies, recombinant proteins, DNA, RNA and virus like particles (VLPs), the Octet platform has become a "must" have laboratory tool for a broad range of biomolecular interactions studies.

The Pioneer platform is a high sensitivity system that incorporates dynamic injection SPR (diSPR®) for improved biophysical characterization of small molecule:protein interactions. Fragment screening campaigns are streamlined with the diSPR method, OneStep®, which determines fragment affinity from the primary screen, saving weeks of instrument and analyst time. The Pioneer system gives more information about small molecule interactions in shorter time, making it a game-changing tool in the drug discovery lab.

Kinetic characterization data
 

Kinetic Characterization
The kinetic characterization of biomolecular interactions to derive on- and off-rates and affinity constants is critical to the screening and selection of desirable products throughout the development and production of therapeutics. With a dip and read label-free technique capable of detecting target molecules in their crude matrix, Pall ForteBio's BLI-based systems are robust and easy to learn. They enable expanded kinetics analyses in a workflow that provides higher throughput than comparable label-free technologies without the limitations of flow-cell based systems. Moreover, they are capable of analyzing the interactions between diverse biologics including in their crude matrix.
 

Dip and Read ELISA
 

Dip and Read ELISA
The Octet platform, with its industry-leading label-free Dip and Read format and ease-of-use, offers an excellent replacement for assays currently performed using ELISA. ELISA's variable results, susceptibility to human error, labor intensive workflow, and slow time-to-result hinder its users. The Octet platform, with its industry-leading label-free Dip and Read format and ease-of-use, offers an excellent replacement for assays currently performed using ELISA. The Octet systems' excellent precision reduces the number of replicates per sample, and high-sensitivity instruments such as the Octet K2, Octet RED96e, Octet RED96, Octet RED384 or Octet HTX offer a dynamic range of >5 logs, essentially eliminating the need to dilute samples.
 

Protein quantitation data
 

Protein Quantitation in Bioprocessing
Accurately determining the concentration of antibodies and other proteins is critical to the selection of cell lines for development and the optimization of antibody production. Rapid turnaround time of results improves process efficiencies and productivity that cannot be achieved with existing methods such as HPLC and ELISA.
 

Small molecule data
 

Small Molecule and Small Peptides
The Pioneer FE system enables combined screening and characterization for small molecule applications using the diSPR technique, OneStep. OneStep determines kinetics and affinity of binding interactions from a single gradient injection. The high sensitivity of Pioneer FE coupled with OneStep/NeXtStep technology makes Pioneer the go-to system for small molecule drug discovery. The Octet RED96e, Octet RED384 and Octet HTX systems offer high sensitivity that enable the analysis of small molecules in a flexible Dip and Read platform that is easy to learn and operate.
 

Assay development data

Assay Development
Octet systems allow users to quickly screen different conditions to select optimal assay conditions during kinetic characterization and process development. Performing efficient assay optimization before proceeding to large-scale implementation saves time, materials, and money. Parallel analysis across eight to sixteen channels without microfluidics allows a multitude of conditions to be assayed in parallel with minimal sample purification and preparation.
 

Literature References

References to Pall ForteBio's products in published literature.