Lead Identification

 

Octet® systems are ideally suited for screening-based lead identification, whether the samples are derived from primary hybridoma hits or from phage display libraries. Early identification of candidates with promising affinities and dissociation kinetics is a powerful strategy for avoiding later-phase failures caused by non-ideal binding characteristics.

Hybridoma Screening

Octet systems enable high-throughput screening of crude hybridoma supernatants for mAb expression levels and binding kinetics. The system contains no microfluidics, making it robust and well-suited for automated analysis of crude matrices. Association and dissociation rates can be estimated directly from supernatant samples, enabling early identification of the most promising clones. View related articles.

Affinity Ranking

Library screening by ELISA does not enable ranking of antibodies based upon their affinities for an antigen. With the Octet system, clones with high affinities and low off-rates can be rapidly identified and selected for further characterization. Many biotechnology companies utilize the Octet system in automated affinity screening and off-rate screening of positive clones obtained from ELISA-based primary screens. View related articles.

Phage Display

Due to its high-throughput design, the Octet system is routinely used as a secondary screening platform for Fab fragments and non-antibody ligands derived from phage display libraries. Using immobilized antigen, an Octet system screen can provide affinity ranking data and estimates of association and dissociation constants for each primary hit. View related articles.