Lead Optimization

 

Label-free kinetic characterization data drive the drug optimization process, guiding the evolution of secondary screening hits into true lead candidates. Octet® systems have established a ubiquitous presence in lead optimization laboratories because they dramatically accelerate the workflow for optimizing engineered antibodies and other biotherapeutic candidates.

Kinetic Characterization

Octet systems generate highly-precise data on molecular binding affinities, specificities, and association/dissociation rates, all at unmatched levels of throughput. These systems are ideally suited for determination of KD, ka, and kd for large panels of molecules. View related articles.

Affinity Maturation

Antibody engineers employ protocols based on mutagenesis or shuffling to achieve low picomolar antibody affinities. These affinity maturation techniques are combinatorial in nature and require kinetic characterization of panels of structural variants – an ideal application for the Octet system's high- throughput format. View related articles.

Epitope Binning

Epitope binning studies rely on the sequential binding of two antibodies to an antigen, and are performed using dozens of antibody pairs in cross-competition matrices. While ELISA assays are sometimes used for epitope binning experiments, they remain labor- and time-intensive and can produce highly variable results. The Octet system excels at these large-scale studies due to assay speed, throughput, and exceptional reproducibility. View related articles.