Dip and Read Biosensors and Assays

Ready-To-Use

Biosensors

Octet® biosensors and assay kits are configured in a standard 96-well format, for your convenience. They are compatible with all Octet instruments (whether
using 96- or 384-well plates). Both kits and biosensors simplify quantitation and kinetics assays by eliminating throughput limitations caused by microfluidic flow, surface regeneration, and sample purification.

Biosensors

ForteBio biosensors are coated with a proprietary biocompatible matrix that is uniform and non-denaturing with minimal non-specific binding. The tips are derivatized and ready for use in a diverse set of biomolecular applications. Biosensors can also be customized for unique applications. Multiple layers can be bound and detected, opening a vast array of approaches to detecting and characterizing biological interactions. To date, over 2.5 million biosensor assays have been run on Octet systems, attesting to the high reproducibility, accuracy, precision and robustness of the platform. Octet biosensors are manufactured in a ISO 9001:2008 certified facility.

Each type of Octet Biosensor is designed to analyze specific biomolecular interactions. A range of surface chemistries is available. Refer to the tables below to select the appropriate biosensor for your needs.

Biosensor Selection Guide

Biosensor Description
AHC - Anti-Human Fc Capture Immobilization of human IgG or other human Fc-containing proteins by binding to the human Fc region.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation. Learn more: download the Anti-human IgG Fc Quantitation (AHQ) Biosensors datasheet
AHQ - Anti-Human IgG Quantitation Binds specifically to the Fc portion of human IgGs and other proteins containing a human Fc region.  Applications include quantitation of human IgG and proteins containing the human Fc region in cell line development, clone selection, process optimization, and production monitoring.
AMC - Anti-Mouse Fc Capture Binds the Fc portion of IgG1, IgG2a, IgG2b for capture based immobilization. Applications include kinetic analysis of antibody-antigen interactions (ka, kd, KD), and off-rate screening. IgG3 should be evaluated on a case-by-case basis. Learn more: download the Anti-Mouse IgG Fc Capture (AMC) Biosensors Datasheet
AMQ - Anti-Murine IgG Quantitation Binds specifically to the Fv(ab')2 portion of mouse and rat IgGs. Applications include quantitaiton of mouse and rat IgG in cell line development, clone selection, process optimization, and production monitoring.
APS - Aminopropylsilane Kinetic applications include adsorption of proteins and membrane fractions through hydrophobic moieties for kinetic screening and affinity characterization (ka, kd, KD). Learn more: download the [Unknown document (id=64)]
AR2G - Amine Reactive 2nd Generation Second-generation carboxylate functionalized surface allows covalent coupling of proteins via EDC/s-NHS mediated amide bond formation.  The second-generation surface provides increased loading density, more robust loading conditions and decreased non-specific binding compared to the original AR biosensor for most proteins. Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), and hit validation. Requires AR2G Assay Kit (part no. 18-5095). Learn more: download the Amine Reactive Second-Generation (AR2G) Biosensor datasheet and Technical Note 26: Dip and Read Amine Reactive Second-Generation (AR2G) Biosensors
FAB2G - Anti-Human Fab-CH1 2nd Generation Binds specifically to the CH1 domain of human IgG, for quantitation and kinetic characterization of human Fab and IgG with improved performance over original FAB biosensors. No binding to free light chains. Learn more: download the Dip and Read Anti-Human Fab-CH1 2nd Generation Biosensors Datasheet and Technical Note 44: Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors
GST - Anti-GST High affinity anti-GST antibody enables direct and rapid quantitation of GST-tagged biomolecules and easy and reliable capture of GST-tagged biomolecules for rapid kinetic analysis. Learn more: download the Anti-GST (GST) Biosensor datasheet , Technical Note 29: GST Biosensor Kinetics Assays and Technical Note 30: GST Biosensor Quantitation Assays
HIS1K - Anti-Penta-HIS Uses the Qiagen Penta-His antibody to capture His-tagged recombinant proteins with high affinity and specificity for either quantitation or kinetic analysis. Learn more: download the Anti-Penta-HIS (HIS1K) Biosensors Datasheet
HIS2 - Anti-HIS Coated with a monoclonal anti-HIS antibody for direct capture and detection of HIS-tagged recombinant proteins for quantitation measurements. Learn more: download the Anti-HIS (HIS2) Biosensor Datasheet and Technical Note 37: Anti-HIS Biosensor Quantitation Assays
NTA - Ni-NTA Nickel charged tris-NTA with strong binding to HIS-tagged recombinant proteins for kinetic measurements and quantitation. Learn more: download the Ni-NTA (NTA) Biosensor datasheet
ProA - Protein A Binds with high affinity to the Fc region of human IgGs.  Binds with moderate affinity to many subtypes of mouse and rabbit IgG. Applications include quantitation of IgG in cell line development, clone selection, process optimization, and production monitoring.
ProG - Protein G Binds with high affinity to the Fc region of murine, rat, goat, and bovine IgGs. Binds with moderate affinity to many subtypes of human IgG.  Applications include quantitation of IgG in cell line development, clone selection, process optimization, and production monitoring.
ProL - Protein L Binds with high affinity to most mouse, rat and human immunoglobulins containing a kappa light chain. Does not bind goat, bovine, rabbit, sheep IgG. Applications include quantitation of FAb fragments and of IgG in serum based culture. Learn more: download the Protein L (ProL) Biosensors datasheet
SA - Streptavidin Streptavidin-coated biosensors for immobilization of biotinylated antibodies, proteins, peptides, or nucleic acids for kinetic applications such as ligand binding analyses, affinity screening, hit validation, epitope binning, and kinetic characterization for rate constant (ka, kd, KD), determination. Learn more: download the Streptavidin (SA) Biosensors datasheet
SAX - High Precision Streptavidin Streptavidin-coated biosensors specifically developed and qualified for applications in drug discovery and quality control labs with stringent requirements for assay precision and robustness in quantitation and binding kinetics characterization assays. QC-tested to meet precision-controlled coefficient of variation (CV) specification of <4% (actual performance in a customer's assay may vary depending on assay conditions). Learn more: download the High Precision Streptavidin (SAX) Biosensor datasheet
SSA - Super Streptavidin Biosensors coated with ultra high density of streptavidin for immobilization of proteins, peptides, nucleic acids, and small molecules. Specifically designed and developed for small fragment screening and small molecule analyses. Learn more: download the Super Streptavidin (SSA) Biosensors datasheet

Literature References

References to ForteBio's products in published literature.

Suggest a Biosensor

Ideas for new biosensors are always welcome. Use our biosensor suggestion form to send your recommendation for a new biosensor offering to the ForteBio product development team.

Don't see the biosensor chemistry you need? Visit our Custom Services web page to find out how ForteBio can make a biosensor just for you.