Dip and Read™ Biosensors and Assays

Ready-to-Use

Octet biosensors and assay kits are configured in a standard 96-well format, for your convenience. They are compatible with all Octet instruments (whether using 96- or 384-well plates). Both kits and biosensors simplify quantitation and kinetics assays by eliminating throughput limitations caused by microfluidic flow, surface regeneration, and sample purification.

Biosensors

ForteBio biosensors are coated with a proprietary biocompatible matrix that is uniform and non-denaturing with minimal non-specific binding. The tips are derivatized and ready for use in a diverse set of biomolecular applications. Biosensors can also be customized for unique applications. Multiple layers can be bound and detected, opening a vast array of approaches to detecting and characterizing biological interactions. To date, over 2.5 million biosensor assays have been run on Octet systems, attesting to the high reproducibility, accuracy, precision and robustness of the platform. Octet biosensors are manufactured in a ISO 9001:2008 certified facility.

Each type of Octet Biosensor is designed to analyze specific biomolecular interactions.  A range of surface chemistries is available. Refer to the tables below to select the appropriate biosensor for your needs.

Biosensor Selection Guide

Biosensor Description Anti-human IgG Fc Binds specifically to the Fc portion of human IgGs and other proteins containing a human Fc region.  Applications include quantitaiton of human IgG and proteins containing the human Fc region in cell line development, clone selection, process optimization, and production monitoring. Download Anti-Human IgG (Fc specific) Biosensors datasheet Anti-murine IgG Fv Binds specifically to the Fv(ab')2 portion of mouse and rat IgGs.  Applications include quantitaiton of mouse and rat IgG in cell line development, clone selection, process optimization, and production monitoring. Download Anti-Murine IgG Fv Biosensors datasheet Protein A Binds with high affinity to the Fc region of human IgGs.  Binds with moderate affinity to many subtypes of mouse and rabbit IgG.  Applications include quantitaiton of IgG in cell line development, clone selection, process optimization, and production monitoring. Download Protein A Biosensors datasheet Protein G Binds with high affinity to the Fc region of murine, rat, goat, and bovine IgGs.  Binds with moderate affinity to many subtypes of human IgG.  Applications include quantitaiton of IgG in cell line development, clone selection, process optimization, and production monitoring. Anti-Penta-HIS (HIS) Uses the Qiagen Penta-HIS antibody to bind with high affinity HIS taged recombinant proteins. Download Anti-Penta-HIS (HIS) Biosensors datasheet Streptavidin (SA) Streptavidin-coated biosensor with high binding capacity.  Immobilizes biotinylated antibodies, proteins, and nucleic acids to form a stable surface.  Quantitation applications include antibody and protein quantitation in cell line development, clone selection, process optimization, and production monitoring.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation. Download Streptavidin (SA) Biosensors datasheet Super Streptavidin (SSA) Streptavidin-coated biosensor with a very high density of biotin binding sites.  Immobilizes biotinylated proteins, peptides, nucleic acids and small molecules to form a stable surface.  Kinetic applications include antibody, protein, peptide, and small molecule kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation. Download Super Streptavidin (SSA) Biosensors datasheet Amine Reactive (AR) Amine functionalized surface to allow covalent coupling of proteins via EDC/NHS mediated amide bond formation.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), and hit validation. Download Amine Reactive (AR) Biosensors datasheet Aminopropylsilane (APS) Adsorption of proteins and membrane fractions through hydrophobic moieties.  Kinetic applications include Adsorption of proteins and membrane fractions through hydrophobic moieties for kinetic screening and affinity characterization (ka, kd, KD). Download Aminopropylsilane (APS) Biosensors datasheet Anti-hIgG Fc Capture (AHC) Immobilization of human IgG or other human Fc-containing proteins by binding to the human Fc region.  Kinetic applications include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit validation. Download Anti-human IgG Fc Capture (AHC) Biosensors datasheet

Octet Biosensor Characteristics

Anti-human IgG Fc Anti-murine IgG Fv Protein A Protein G HIS SA SSA AR APS AHC Applications:    Quantitation (Q)    Screening (S)    Kinetics (K) Q Q Q Q Q Q, S, K Q, S, K S, K K K Surface can be regenerated* NO NO YES YES NO YES YES YES YES YES For small molecules (150-900 Da) on Octet RED For peptides (500-2000 Da) on Octet RED For small proteins (5-20 kD) For mid sized proteins (20-150 kD) For large proteins (>150 kD) Short kinetic assays (off rate <15 min) Long kinetic assays (off rate >15 min) Custom quantitation assays LOD <50 ng/mL, requires fast flow-rate and longer assay time Upper limit of quantitation >1 mg/mL Quantitation in serum-free crude cell lysates Quantitation in serum- containing crude cell lysates Quantitation in column elutes, buffer High detergent concentrations (>1%)  = Not Advised      = Good      = Very Good      = Exellent *Dependant on chemistry of protein attached to the biosensor.

Literature References

References to ForteBio's products in published literature.

Suggest a Biosensor

Ideas for new biosensors are always welcome. Use our biosensor suggestion form to send your recommendation for a new biosensor offering to the ForteBio product development team.

Don't see the biosensor chemistry you need? Visit our Custom Services web page to find out how ForteBio can make a biosensor just for you.

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