Biosensor Types

Ready-to-Use

Octet biosensors are configured in a standard 96-well format. Biosensors simplify quantitation and kinetics by eliminating throughput limitations caused by microfluidic flow, surface regeneration, and sample purification.

Octet biosensor tips are coated with a proprietary biocompatible matrix that is uniform, non-denaturing, and has minimal non-specific binding. The tips are derivatized and ready for use in specific biomolecular applications. Various molecules can be attached to the surface, and multiple layers can be bound to study a myriad of biological interactions.

Each type of Octet Biosensor is designed to analyze specific biomolecular interactions. A range of surface chemistries is available, with choices of grade and binding capacity. Refer to the table below to select the appropriate grade for your needs:

Octet Biosensor Grades

Grade	Description
Quantitation Grade	Ready to use for protein quantitation based on a standard calibration or yes/no binding
Screening Grade	Kinetic screening applications with off-rates < 15 minutes. Also ideal for custom quantitation assays.
Kinetics Grade	Kinetic characterization applications with baselines stable for 1 hour. Can be used for custom quantitation assays.

Octet Biosensor Surface Chemistries

Surface Chemistry	Available Grades	Defining Characteristics
Amine Reactive	Screening Kinetics	Covalently couples any protein of interest with a primary amine to measure binding and kinetic characterization.
Super Streptavidin	Screening Kinetics	Streptavidin coated biosensor for small molecule analysis on the Octet RED System. Covalently bind small molecules or small peptides.
Streptavidin High Binding	Screening Kinetics	Streptavidin coated biosensor with a higher binding capacity than the standard binding capacity. Ideal for applications involving custom quantitation and the screening of FAbs, peptides and smaller proteins.
Streptavidin SBC (Standard Binding Capacity)	Screening Kinetics	Streptavidin-coated biosensors for covalent binding of biotinylated proteins.
Protein A	Quantitation	Protein A Biosensors provide concentration determination typically within the range of 1–500 µg/mL.
Anti-murine IgG	Quantitation	Anti-mIgG F(ab')2 biosensors provide concentration determination for either mouse or rat IgG samples.
Anti-human IgG FC-Specific	Quantitation	Anti-human IgG, Fc Specific Biosensors provide concentration determination typically within the range of 1–100 µg/mL.
Aminopropylsilane (APS)	Kinetic	Hydrophobic or electrostatic immobilization of most proteins.

Biosensor Selection

Quantitation

Octet comparison chart

Kinetics

Factor

SBC
(Kinetics Grade)

SBC FA
(Screening Grade)

HBC
(Kinetics Grade)

HBC FA
(Screening Grade)

Super SA
(Kinetics Grade)

Amine Reactive

APS

Binding capacity

S/N for small molecules (300-900 Daltons) on Octet RED

S/N for peptides (500-2000 Daltons) on Octet RED

S/N for small proteins (5-20kD)

S/N for mid sized proteins (20-150kD)

S/N for large proteins (>150kD)

Short kinetics assays (off rate <15min

Long kinetics assays (off rate >15min)

Applications at high detergent concentrations (>1%)

Immobilization of non-biotinylated proteins

Custom quantitation

Epitope mapping/binning

Regeneration capable

= Not Advised

= Good

= Very Good

= Exellent

Suggest a Biosensor

Ideas for new biosensors are always welcome. Use our biosensor suggestion form to send your recommendation for a new biosensor offering to the ForteBio product development team.

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