In This Issue
Fast and Easy Generic Anti-CHO HCP Analysis: 96 Samples, Assay to Data in 65 Minutes with the NEW Anti-CHO HCP Detection Kit
Darick Dayne, PhD, Senior Product Manager, firstname.lastname@example.org
Pall ForteBio LLC and Cygnus Technologies recently teamed up to jointly develop our new Anti-CHO HCP Detection Kit for quantitation of residual HCPs. Cygnus HCP ELISA kits are well known for their broad HCP recognition and sensitivity, and combining their industry-standard 3G anti-CHO HCP antibody with the Octet® platform's easy and rapid high-throughput protein analysis gives you the best of both worlds for HCP assays!
LISA is the most commonly used HCP analysis method used today, but it requires multiple time-consuming incubation steps and relies on highly manual processing that introduces a lot of data variability. Octet systems can now automate the entire HCP assay process for you. Minimal hands-on time is needed and assay precision is improved by as much as 15% with better or equivalent sensitivity and dynamic range. A completely hands-off, walk-away HCP assay run on the Octet HTX system can give you fully analyzed results for 96 samples in one hour — the same assay run with ELISA can take about 3 hours. HCP assays can also be run on Octet RED384 and RED96 systems using the Sidekick™ Station with results in 75–90 minutes.
The Octet platform can be used for generic CHO HCP assays in early phases of clinical product development, and process-specific CHO HCP assays can also be constructed using the same assay format if a process-specific antibody has been developed. And because you can now monitor HCP data in real time, you'll be able to greatly reduce your assay development times as well.
We launched the new ForteBio-Cygnus Anti-CHO HCP Detection Kit in August and the initial feedback from customers is spectacular! Here's what one researcher from a US biotech firm on the east coast told us:
"The (Octet assay) sensitivity is quite a bit better than ELISA methods and there was no interference from our protein at 1 mg/mL. With other ELISA methods I have had to dilute to 0.2 mg/mL so the increase in sensitivity is 5-fold over that of ELISA. In addition, it would be fairly easy to adapt the technology to in- house produced antibodies against specific cell lines."
|Assay Performance||Cygnus 3G ELISA Kit||ForteBio-Cygnus Anti-CHO HCP Detection Kit|
|Time-to-Result||210 min||65 min on Octet HTX system|
|75 min on Octet RED384 system with Sidekick Station|
|90 min on Octet RED96 system with Sidekick Station|
|Dynamic Range||1–100 ng/mL||0.5–200 ng/mL|
|Sample Set||Drug Titer
HCP ELISA (ppm)
|HCP Assay on Octet
|Cygnus 3G ELISA||HCP Assay on Octet Platform|
|In-process Sample 1||10000
|In-process Sample 2||500||4577||4.0%||100
|In-process Sample 3||20||41||9.4%||10
Get all of the above with the new Anti-CHO HCP Detection Kit from Pall ForteBio LLC. We'll also throw in higher assay drug tolerance, sensitivity down to 0.5 ng/mL and a gold-standard Cygnus Technologies 3G antibody for the broadest reactivity you can get. When you run HCP assays on an Octet system, you'll have fully analyzed results in a third of the time it takes with ELISA, and not have to babysit a single step. That means shorter development times and higher quality data that's off to regulatory agencies sooner. Can't get sweeter than that!
fortebio.com | 888-OCTET-75