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ForteBio Interactions Newsletter Biosensor photo

January 2009    VOLUME 2    ISSUE 1

WWW.FORTEBIO.COM

 

Getting a Lot More Done... with Less

Chris Silva
Vice President, Marketing

In each issue, a member of the executive team is invited to communicate with our readers, and I am pleased to be contributing to the January 2009 issue of Interactions.

First, I want to thank all our customers and users of the Octet® platform for supporting us through 2008. At ForteBio, we experienced significant growth during 2008, above and beyond our peers. This enabled us to build a great company that can fund development programs to further enhance our capabilities. Also, during this past year, we expanded our sales and support footprint by hiring employees in Europe, and by placing instruments with our distributors and/or customers in Japan, China, Australia, and India.

One might ask why specifically were we successful during 2008. To me, the answer lies in our Octet platform’s unique ability to dramatically increase productivity in the lab. For example, consider a traditional ELISA assay that is run to determine antibody titers for clone selection in biotherapeutic development. Our customers have shown greater than 10X savings in hands-on time and turnaround time.

With label-free Octet assays, there are no fluorophore or chromophore conjugation steps, thereby eliminating many of the incubation steps and completely eliminating the need to wash microplate wells. The time to result for a typical quantitation assay on an Octet instrument is 30 minutes for a 96-well plate. Further, similar productivity improvements are also available for customers running kinetic characterization studies (kobs, ka, kd, KD) and are discussed on our web site.


Looking at 2009, I feel the Octet platform’s capabilities are going to be even more important in enabling scientists to get more done with less. It is likely that your lab needs to run the same number of assays—or more—with a smaller budget than in 2008. The Octet platform can help accomplish just that. I wish all of our customers and strategic partners’ success in achieving their 2009 goals, and look forward to expanding the ForteBio toolset of offerings during the coming year.

ForteBio Biosensor Selection Guide

Factor

Anti-Human lgG Fc

Anti-Murine lgG FC

Protein A

SA

SSA

AR

APS

APS

Application:
Quantitation(Q), Screening(S), Kinetics(K)

Q

Q

Q

Q,S,K

Q,S,K

S,K

K

K

Surface can be regenerated*

NO

NO

YES

YES

YES

YES

YES

YES

For small molecules (150-900 Da) on Octet RED

Not Advised

Not Advised

Not Advised Not Advised Excellent Not Advised Good Not Advised

For peptides (500-2000 Da) on Octet RED

Not Advised Not Advised Not Advised Good Excellent Not Advised Good Good

For small proteins (5-200 kDa)

Not Advised

Very Good

Excellent Excellent Excellent Good Good Very Good

For mid-sized proteins (20- 150 kDa)

Very Good

Excellent

Excellent Excellent Excellent Very Good Excellent Excellent

For large proteins (>150kDa)

Excellent

Excellent

Excellent Excellent Excellent Very Good Excellent Excellent

Short kinetics assays (off rate <15 mins)

Not Advised

Not Advised

Not Advised Excellent Excellent Very Good Excellent Excellent

Long kinetics assays (off rate >15mins)

Not Advised Not Advised Not Advised Excellent Excellent Very Good Very Good Excellent

Custom Quantitation assays

Not Advised Not Advised Not Advised Excellent Good Not Advised Not Advised Not Advised

LOD <50ng/ml, requires fast flow-rate and longer assay time

Very Good Very Good Very Good Very Good Not Advised Not Advised Not Advised Not Advised

Upper limit of quantitation > 1mg/ml

Not Advised Not Advised Very Good Very Good Not Advised Not Advised Not Advised Not Advised

Quantitation in serum-free crude cell lysates

Excellent Excellent Excellent Excellent Not Advised Not Advised Not Advised Not Advised

Quantitation in serum-containing crude cell lysates

Excellent Excellent Good Excellent Not Advised Not Advised Not Advised Not Advised

Quantitation in column elutes, buffer

Excellent Excellent Excellent Excellent Not Advised Not Advised Not Advised Not Advised

High detergent concentrations (>1%)

Excellent Excellent Excellent Excellent Excellent Good Excellent Good
Not Advised

 = Not Advised    

Good

 = Good    

Very Good

 = Very Good    

Excellent

 = Excellent

*Dependant on chemistry of protein attached to the biosensor

Recent Publication References: Octet Platform

Advantages and application of label-free detection assays in drug screening.
B.T. Cunningham et .al., Expert Opinion on Drug Discovery, 3(8): 891-901, 2008

Human antibodies to human delta-like ligand 4.
N.J. Papadopoulos et. al., Regeneron Pharmaceuticals, Inc., United States Patent Application 20080181899

Culture method for obtaining a clonal population of antigen-specific B cells.
J. Carvalho et .al., UCB, United States Patent Application 20070269868

High affinity human antibodies to human IL-4 receptor.
S. Stevens et. al., Regeneron Pharmaceuticals, Inc., United States Patent Application 20080260035

Humaneered anti-factor B antibody.
W. Emlen et. al., Taligen Therapeutics, Inc., United States Patent Application 20080299114

WNT antagonists and their use in the diagnosis and treatment of WNT-mediated disorders.
J. A. Ernest et. al., Genentech, Inc. United States Patent Application 20080299136

The surface protein TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells.
L.C. Gonzalez et. al., Genentech, Inc., Nature Immunology, 10:48-57, 2008.

 

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