New Anti-Penta-HIS Biosensor for Quantitating HIS-Tagged Proteins

Bettina Heidecker, Scientist

The six-histidine or penta-histidine peptide tag (HIS-tag) is a common tag fused to recombinant proteins during cloning. Many tools have been developed for detection and purification of HIS tagged-proteins. The Anti-Penta-HIS biosensor, for use on the Octet system, provides a rapid, label-free one-step quantification method of HIS-tagged proteins. This new biosensor uses the highly specific Anti-Penta-HIS antibody from Qiagen pre-immobilized onto the biosensor to capture these proteins (Figure 1).

Protein quantitation utilizing the HIS-tag can be used in many steps of a protein production process, since the biosensor is compatible with column eluates, cell culture supernatant, and crude cell lysates. Sample dilution, depending on the crude matrix, is recommended. Proteins of different molecular weight can be quantified with a typical dynamic assay range from 0.25–200 µg/mL depending on the specific protein and the assay conditions, if run using the standard or high sensitivity assay protocols built into the latest version of the Octet software. Analysis of the binding data (Figures 2A and 2B) is straightforward using the Octet data analysis software, which offers many choices of binding rate calculations, enabling quantitation of a wide variety of HIS-tagged proteins.

For more information, or to order Anti-Penta-HIS biosensors, please email info@fortebio.com or visit our biosensors page.