Octet^® RED384 and Octet QK384 System Specifications

SAMPLE AND ANALYSIS
Detection Technology	Bio-Layer Interferometry (BLI)
Biosensor Type	Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking
Information Provided	Kinetic and affinity analysis —k_obs, k_a, k_d, K_D Kinetic screening for k_a or k_d Binding specificity and cooperativity Concentration monitoring of real-time binding (no need for background subtraction) Automated concentration determinations
Data Presentation	Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts
Sample Types	Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Sample Plate	Standard, 96-well and 384-well, black, flat-bottom microplate
Sample Volume	80–130 µL/well (384-well plate) and 180–220 µL/well (96-well plate) Nondestructive testing, easily recovered
Orbital Flow Capacity	Static or 100–1500 rpm
Analysis Temperature Range	(Ambient + 4 °C) – 40 °C, 1 °C increments)
QUANTITATION AND KINETICS
Throughput	Up to 16 assays in parallel; up to 96 assays per 96-well plate and 384 assays per 384-well plate
Analysis Time per Sample	hIgG quantitation in 2 minutes for 16 samples, ≤20 minutes for 96 samples and ≤75 minutes for 384 samples Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
Baseline Noise	< 3 pm (RMS) for Octet RED384 < 6 pm (RMS) for Octet QK384
Quantitation Range for hIgG	Octet RED384: 0.5 µg/mL–2000 µg/mL at 400 rpm, 0.05 µg/mL–100 µg/mL at 1000 rpm Octet QK384: 1 µg/mL–700 µg/mL at 400 rpm, 0.1 µg/mL–100 µg/mL at 1000 rpm
PHYSICAL SPECIFICATIONS
Dimensions	30.1" H x 31.5" W x 31.5" D (77 cm H x 80 cm W x 80 cm D)
Weight	150 lb (68.2 Kg)
Electrical Requirements	Mains: AC 100–240 V, 5.0–2.0A, 50/60Hz, single phase Power consumption: 195w (240 peak)
Safety Standards	CE, CSA

Octet® RED384 and Octet QK384 System Specifications

Octet^® RED384 and Octet QK384 System Specifications