Octet® K2 System

Octet HTX System

Octet K2 System

The Octet K2 2-channel system brings you unprecedented access to exquisite biomolecular interactions data. Priced for start-up academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 system characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multi-purpose Octet K2 system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates.

Key Features

  • Sensitivity for small molecule analysis
  • Measure interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates
  • Dual channels, dual spectrometers
  • Unique 2-channel design with dedicated spectrometers for simultaneous referencing or added capacity
  • Simple software setup
  • Template driven interface to get new users started quickly

Kinetic Analysis

The Octet K2 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and dissociation constants (KD). The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference. Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay.

With pre-defined templates to follow in Octet Data Acquisition software, the Octet K2 system streamlines setup prior to running an assay and minimizes training needs. Octet Data Analysis software then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface.

Large molecule characterization. Example data from human Prostate Specific Antigen (PSA, MW 30 kDa) binding to a biotinylated anti-human PSA mouse monoclonal antibody loaded onto Streptavidin biosensors. Binding was performed at 30 C, with a shake speed of 1000 rpm. A 200 nM PSA solution was prepared and serial diluted 1:2 to obtain the 5 concentrations run.

Figure 1: Large molecule characterization. Example data from human Prostate Specific Antigen (PSA, MW 30 kDa) binding to a biotinylated anti-human PSA mouse monoclonal antibody loaded onto Streptavidin biosensors. Binding was performed at 30°C, with a shake speed of 1000 rpm. A 200 nM PSA solution was prepared and serial diluted 1:2 to obtain the 5 concentrations run.

Small molecule kinetics. Example data from benzenesulfonamide (MW 157 Da) binding to biotin-carbonic anhydrase loaded on Super Streptavidin biosensors. Binding was performed at 30°C, with a shake speed of 1000 rpm.  A 100 µM benzensulfonamide solution was prepared and serial diluted 1:4.

Figure 2: Small molecule kinetics. Example data from benzenesulfonamide (MW 157 Da) binding to biotin-carbonic anhydrase loaded on Super Streptavidin biosensors. Binding was performed at 30°C, with a shake speed of 1000 rpm. A 100 µM benzensulfonamide solution was prepared and serial diluted 1:4.

Quantitative Measurements

Utilizing Pall ForteBio's one-step Dip and Read assays, the Octet K2 system directly measures the presence of specific proteins and other molecules in solution. Accurate and reproducible concentrations can be determined in as little as 2 minutes per sample.

Biosensor Re-racking

The Octet K2 system provides the ability to re-rack biosensors at the end of an experiment. Biosensor re-racking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.

Literature References

References to Pall ForteBio's products in published literature.