Octet^® RED96 System Specifications

SAMPLE AND ANALYSIS
Detection Technology	Bio-Layer Interferometry (BLI)
Biosensor Type	Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking
Information Provided	Kinetic and affinity analysis (k_obs, k_a, k_d, K_D) Concentration monitoring (no need for background subtraction) Automated concentration determinations
Data Presentation	Plots displaying kinetic binding, equation fits, and residuals of fits Tabulated kinetic data and data charts One-click concentration data analysis including generation of calibration curves and output of tabulated concentration data
Sample Types	Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Sample Plate	Standard, 96-well, black, flat-bottom microplate
Sample Volume	180–220 µL/well Nondestructive testing
Orbital Flow Capacity	Static or 100–1500 rpm
Analysis Temperature Range	(Ambient + 4°C) – 40°C, 1°C increments
QUANTITATION AND KINETICS
Throughput	Up to 8 assays in parallel; up to 96 assays per 96-well plate
Analysis Time per Sample	hIgG quantitation in 2 minutes for 8 samples, ≤32 minutes for 96 samples Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
Quantitation Range for hIgG	0.5 µg/mL to 2000 µg/mL at 200 rpm, 0.05 µg/mL to 100 µg/mL at 1000 rpm
Baseline Noise	≤3 pm (RMS)
PHYSICAL SPECIFICATIONS
Dimensions	18.6 in (H) x 17 in (D) x 20.8 in (W) 47 cm (H) x 43 cm (D) x 53 cm (W)
Weight	63 lb (28.6 Kg)
Data Handling and Storage	Mains: AC 100–240 V, 5.0–2.0 A, 50/60 Hz, single phase Power consumption: 120W (240 peak)
Safety Standards	CE and CSA

Octet® RED96 System Specifications

Octet^® RED96 System Specifications