SAMPLE AND ANALYSIS |
Detection Technology |
Bio-Layer Interferometry (BLI) |
Biosensor Type |
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking |
Information Provided |
- Kinetic and affinity analysis —kobs, ka, kd, KD
- Kinetic screening for ka or kd
- Binding specificity and cooperativity
- Concentration monitoring of real-time binding (no need for background subtraction)
- Automated concentration determinations
|
Data Presentation |
- Plots displaying kinetic binding, equation fits, and residuals of fits
- Tabulated kinetic data and data charts
|
Sample Types |
Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
Sample Plate |
Standard, 96-well and 384-well, black, flat bottom, microplate |
Sample Volume |
- 80–120 μL/well (384-well plate) and 180–220 μL/well (96-well plate)
- Nondestructive testing, easily recovered
|
Orbital Flow Capacity |
Static or 100–1500 rpm |
Analysis Temperature Range |
(Ambient + 4 °C) – 40 °C, 1 °C increments) |
QUANTITATION AND KINETICS |
Throughput |
Up to 16 assays in parallel; up to 96 assays per 96-well plate and 384 assays per 384-well plate |
Analysis Time per Sample |
- hIgG quantitation in 2 minutes for 16 samples, ≤20 minutes for 96 samples and ≤75 minutes for 384 samples
- Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
|
Baseline Noise |
- < 3 pm (RMS) for Octet RED384
- < 6 pm (RMS) for Octet QK384
|
Quantitation Range
for hIgG |
- Octet RED384: 0.5 μg/mL–2000 μg/mL at 400 rpm, 0.05 μg/mL–100 μg/mL at 1000 rpm
- Octet QK384: 1 μg/mL–700 μg/mL at 400 rpm, 0.1 μg/mL–100 μg/mL at 1000 rpm
|
PHYSICAL SPECIFICATIONS |
Dimensions |
30.1” H x 31.5” W x 31.5” D
(77 cm H x 80 cm W x 80 cm D) |
Weight |
150 lb (68.2 Kg)
|
Electrical Requirements |
Mains: AC 100–240 V, 5.0–2.0A, 50/60Hz, single phase
Power consumption: 195w (240 peak)
|
Safety Standards |
CE, CSA |
