OCTET RED SYSTEM |
Detection Technology |
Biolayer Interferometry (BLI) |
Capabilities |
Kinetic and affinity data—kobs, ka, kd, KD; binding specificity and cooperativity
Epitope mapping/binning
Yes/No binding
Protein concentration determinations |
Sampling Format |
Test plate: 96-well, black, flat bottom, polypropylene microplate
- SBS standard plate
- Single, test plate capacity
Test volume: 100-200 μL
- Application-dependent
- Non-destructive and recoverable
Sample types: low MW analytes, peptides, proteins
Buffer compatibility: cell media, serum, plasma, DMSO |
Analysis Runtime per Interaction |
Variable, up to 3 hours total runtime |
Typical Working Ranges |
Kinetics
- Association rate constant (ka): 101 to 107 M-1 s-1
- Dissociation rate constant (kd): 10-6 to 10-1 s-1
- Affinity range: 10-3 to 10-12 M
Quantitation
- 0.5 μg/mL to 2000 μg/mL
- Protein and assay–dependent
Molecular weight detection: >250 Da
Baseline noise: typically < 2.5 pm RMS
Baseline drift: typically < 21 pm/hour
Analysis temperature range: ambient to 47 °C |
Automation |
Up to 8 biosensors in parallel
Maximum of 96 tests unattended |
Optics and Mechanics |
8-channel biosensor manifold
Optical interferometer
Test plate temperature
- 2°C over ambient to 42°C
- 1°C increment
Orbital flow
|
Dimensions |
Height: 18.6 inches (475 mm)
Depth: 17 inches (430 mm)
Width: 20.8 inches (530 mm)
Weight: 54 lbs. (24.5 Kg) |
Electrical Requirements |
Mains: AC 100 – 240 V, 2.0 – 0.9A, 50/60 Hz, single phase
Power consumption: 120w (240 peak) |
Data Handling and Storage |
Integrated software package for operation and data analysis
PC operating system: Microsoft® Windows® XP (Professional)
Interfacing: RS232, USB |
Compliance |
TBD |
