OCTET^® SOFTWARE

Current Version: V7.0

Octet software continues to rapidly evolve to address the needs of ForteBio customers. Version 7.0 enhances workflow for both quantitation and kinetic analysis with improved data viewing, simplified navigation of large data sets and the ability to remotely monitor experiments on the Octet platform.

Replicate Groups
Organize replicate samples into groups for convenient calculation of average concentration, average binding rate, standard deviation and CV%. Statics are automatically calculated during data analysis and can be plotted in the new X-Y graphing tool for custom viewing.
Accelerated Binding Calculation
Process datasets in a fraction of the time and view final data sooner. An improved and validated algorithm provides the same quality quantitation data — just faster.
Remote Monitoring
Want to observe your data in real time, but unable to stay in front of the Octet instrument? Monitor experiments remotely from any networked computer, smartphone or mobile device using a standard web browser using remote monitoring.
Grouped View (for Quantitation)
One of the most popular features for kinetics analysis is now available for quantitation analysis. Simplify the visual analysis of datasets with highly tailored displays organized by groups.
1:2 Bivalent Analyte Model
Perform a full kinetic analysis of data for bivalent analytes interacting with an immobilized ligand. The bivalent model provides terms for two association constants and two dissociation constants.

FOR DATA ACQUSITION, DATA ANALYSIS AND REPORTING

The Octet system is fully controlled by software that simplifies assay setup, data acquisition, and analysis. An Experiment Wizard assists the operator through each step via simple prompts, facilitating experimental setup, and results are displayed in real time during data collection.

Experiment Wizard guides users through assay setup

Data analysis can be performed either at the instrument or on a remote computer, streamlining the workflow to increase efficiency and minimize operator time. For each experiment, a directory folder is created, in which raw data files for each sensor will be saved. Raw data is always accessible, if archiving is desired.

Users can save operator time and reduce the potential for error by saving experimental method files in advance and recalling at runtime. The software also supports importing tab-delimited text or Notepad files for sensor and sample ID, and concentration/sample/buffer information.

Assay set up in Octet software is as easy as 1-2-3-4.

The software’s programmable dilution factors for unknown samples simplify quantitation during data analysis, while click-and-drag functions simplify assigning well types to multiple wells (e.g., standards, unknowns or controls).

Real-Time Data Acquisition

The Runtime binding view window shows plates' experimental status, sample progress and elapsed time, enabling rapid evaluation of raw data for expected trends and monitoring binding progress. A highly interactive interface makes customizing the assay window easy and intuitive. The user can automate and speed up assay development by setting a threshold value for the binding signal in any step in the experiment. Reference-subtracted data can be viewed in real time — even while data is being acquired.

Data Analysis

The data analysis options are extremely powerful and flexible. An entire experiment, or individual files (sensor raw data) from single or multiple experiments can be selected, to optimize the data analysis and workflow. Users can apply a single set of standards to any number of sample microplates, or, apply calibration for unknowns within each microplate. For kinetic screening and characterization, the software includes 2:1 Heterogeneous ligand interaction analysis. Due to unique circumstances in some binding systems, data may be influenced by mass transport limitation. The Mass Transport fit in the Octet data analysis software enables you to analyze such data to extract true binding constants.

Flexible Data Management Options

After analysis, a complete data report can be assembled from the various charts and tables according to your requirements, then exported as a Microsoft Excel document for easy lab notebook layout and subsequent analysis. Graphs can be exported as jpeg files at any time. Additionally, ForteBio recognizes scientists in life science organizations need to handle low to high volumes of data generated from routine screening on the Octet systems. We are actively working in collaboration with data management software providers such as IDBS to enable users to easily transfer data from the Octet software to databases such as the ActivityBase suite. If you have a specific data management issue or suggestion, please contact support@fortebio.com today.