|
Octet QKe |
Octet QK |
|
Quantitation and Kinetics |
|
Throughput |
Up to 8 assays in parallel; up to 96 assays per 96-well plate |
Up to 8 assays in parallel; up to 96 assays per 96-well plate |
|
Analysis Time per Sample |
- hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
- Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
|
- hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
- Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
|
|
Baseline Noise |
≤ 3 pm (RMS) at High Sensitivity Quantitation setting
≤ 8 pm (RMS) at Standard Quantitation setting |
≤ 20 pm (RMS) |
|
Quantitation Range
for hIgG |
1 µg/mL to 700 µg/mL at low rpm; LOD of 0.05 µg/mL at 1000 rpm on Protein A biosensors |
1 µg/mL to 700 µg/mL on Protein A biosensors |
|
Operational Specifications |
|
Sample Plate |
Standard 96-well, black, flat bottom microplate |
Standard 96-well, black, flat bottom microplate |
|
Sample Volume |
- 180–220 μL/well (96-well plate)
- Nondestructive testing, easily recovered
|
- 180–220 μL/well (96-well plate)
- Nondestructive testing, easily recovered
|
|
Orbital Flow Capacity |
Static or 100–1500 rpm |
Static or 100–1500 rpm |
|
Analysis Temperature Range |
(Ambient + 4 °C) – 40 °C, 1 °C increments |
(Ambient + 4 °C) – 40 °C, 1 °C increments |
|
Re-racking |
Yes; Biosensor re-racking enabled |
Not available |
|
Sample and Analysis |
|
Detection Technology |
Biolayer Interferometry (BLI) |
Biolayer Interferometry (BLI) |
|
Biosensor Type |
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking |
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking |
|
Information Provided |
- Kinetic and affinity analysis
(kobs, ka, kd, KD)
- Kinetic screening for ka or kd
- Binding specificity and cooperativity
- Concentration monitoring of real-time binding (no need for background subtraction)
|
- Kinetic and affinity analysis
(kobs, ka, kd, KD)
- Kinetic screening for ka or kd
- Binding specificity and cooperativity
- Concentration monitoring of real-time binding (no need for background subtraction)
|
|
Data Presentation |
- Plots displaying kinetic binding, equation fits, and residuals of fits
- Tabulated kinetic data and data charts
|
- Plots displaying kinetic binding, equation fits, and residuals of fits
- Tabulated kinetic data and data charts
|
|
Sample Types |
Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
|
Physical Specs |
|
Dimensions |
18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W) |
18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W) |
|
Weight |
54 lb (24.5 Kg) |
50 lb (22.7 Kg) |
|
Electrical Requirements |
- Mains: AC 100–240 V, 5.0–2.0 A,
50/60 Hz, single phase
- Power consumption: 120W (240 peak)
|
- Mains: AC 100–240 V, 5.0–2.0 A,
50/60 Hz, single phase
- Power consumption: 120W (240 peak)
|
