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Octet QKe |
Octet QK |
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Quantitation and Kinetics |
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Throughput |
Up to 8 assays in parallel; up to 96 assays per 96-well plate |
Up to 8 assays in parallel; up to 96 assays per 96-well plate |
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Analysis Time per Sample |
- hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
- Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
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- hIgG quantitation in 2 minutes for 8 samples, ≤ 32 minutes for 96 samples
- Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
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Baseline Noise |
≤ 3 pm (RMS) at High Sensitivity Quantitation setting
≤ 8 pm (RMS) at Standard Quantitation setting |
≤ 20 pm (RMS) |
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Quantitation Range
for hIgG |
1 µg/mL to 700 µg/mL at low rpm; LOD of 0.05 µg/mL at 1000 rpm on Protein A biosensors |
1 µg/mL to 700 µg/mL on Protein A biosensors |
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Operational Specifications |
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Sample Plate |
Standard 96-well, black, flat bottom microplate |
Standard 96-well, black, flat bottom microplate |
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Sample Volume |
- 180–220 μL/well (96-well plate)
- Nondestructive testing, easily recovered
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- 180–220 μL/well (96-well plate)
- Nondestructive testing, easily recovered
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Orbital Flow Capacity |
Static or 100–1500 rpm |
Static or 100–1500 rpm |
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Analysis Temperature Range |
(Ambient + 4 °C) – 40 °C, 1 °C increments |
(Ambient + 4 °C) – 40 °C, 1 °C increments |
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Re-racking |
Yes; Biosensor re-racking enabled |
Not available |
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Sample and Analysis |
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Detection Technology |
Biolayer Interferometry (BLI) |
Biolayer Interferometry (BLI) |
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Biosensor Type |
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking |
Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking |
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Information Provided |
- Kinetic and affinity analysis
(kobs, ka, kd, KD)
- Kinetic screening for ka or kd
- Binding specificity and cooperativity
- Concentration monitoring of real-time binding (no need for background subtraction)
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- Kinetic and affinity analysis
(kobs, ka, kd, KD)
- Kinetic screening for ka or kd
- Binding specificity and cooperativity
- Concentration monitoring of real-time binding (no need for background subtraction)
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Data Presentation |
- Plots displaying kinetic binding, equation fits, and residuals of fits
- Tabulated kinetic data and data charts
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- Plots displaying kinetic binding, equation fits, and residuals of fits
- Tabulated kinetic data and data charts
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Sample Types |
Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates |
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Physical Specs |
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Dimensions |
18.6 in (H) x 17 in (D) x 20.8 in (W) 47 cm (H) x 43 cm (D) x 53 cm (W) |
18.6 in (H) x 17 in (D) x 20.8 in (W) 47 cm (H) x 43 cm (D) x 53 cm (W) |
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Weight |
54 lb (24.5 Kg) |
50 lb (22.7 Kg) |
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Electrical Requirements |
- Mains: AC 100–240 V, 5.0–2.0 A,
50/60 Hz, single phase
- Power consumption: 120W (240 peak)
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- Mains: AC 100–240 V, 5.0–2.0 A,
50/60 Hz, single phase
- Power consumption: 120W (240 peak)
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