SPR Biosensor Surface Chemistries for Pioneer Systems

We offer a selection of surface chemistries for use on the Pioneer and Pioneer FE platforms — from amine reactive surfaces with varying capacities to pre-immobilized capture chemistries. Our high quality SPR biosensors enable researchers to optimize for a diverse array of ligand types and applications, ensuring generation of high quality data from interaction analysis studies.

SPR Biosensor Selection Guide

Biosensor Description
COOH1 The COOH1 biosensor is a low capacity two-dimensional surface chemistry that comprises a polyethylene glycol (PEG) spacer terminated with carboxylic acids. This surface is particularly useful where lectin binding may occur or where Dextran coatings are not desired. Amine coupling is the standard immobilization method for COOH1 and its coupling capacity is the lowest of the carboxyl biosensors. Typical uses are large molecule (>25 kDa) kinetic assays.
COOH2 The COOH2 biosensor is a medium capacity three-dimensional chemistry that comprises a short Dextran spacer with carboxylic acids. The Dextran spacer is excellent for reducing non-specific binding and is biocompatible with a wide range of molecules. Amine coupling is the standard immobilization method for COOH2 and its coupling capacity is between the COOH1 and COOHV biosensors. Typical uses are intermediate (>1 kDa) to large molecule (>25 kDa) kinetics.
COOH5 The COOH5 biosensor is a high capacity three-dimensional chemistry that comprises a carboxylated polysaccharide hydrogel spacer. This surface is useful where a Dextran based hydrogel is not desired. This biosensor contains a high density of carboxylic acid functional groups that allows a large amount of target to be coupled onto the surface. This is particularly useful to observe the interactions of small molecules or for targets that exhibit low binding activity. Amine coupling is the standard immobilization method for COOH5 and its coupling capacity is the highest of the carboxyl biosensors.
COOHV The COOHV biosensor is a high capacity three-dimensional chemistry that uses a medium length Dextran spacer with carboxylic acids. This biosensor contains a high density of carboxylic acid functional groups that allows a large amount of target to be coupled onto the surface. This is particularly useful to observe the interactions of small molecules or for target that exhibit low binding activity. Amine coupling is the standard immobilization method for COOH and its coupling capacity is the higher than the COOH2 biosensors.
HisCap The HisCap biosensor is a three-dimensional hydrogel chemistry derived from the COOH5 biosensor. It is modified with Nitrilotriacetic acid (NTA) functional groups that chelate Nickel ions for the selective capture of 6X and 10X His-tagged targets. This surface can be partially regenerated by injecting Imidazole, EDTA or other similar reagents that compete for Nickel. Typical uses are small molecule or peptide kinetics with His-tagged protein targets.
BioCap The BioCap biosensor is a three-dimensional chemistry derived the COOHV biosensor. It has been preimmobilized with NeutrAvidin biotin binding protein to allow for the selective irreversible capture of biotin containing ligands. The near neutral pI of the NeutrAvidin contributes to the low non-specific binding on this surface. Typical uses are intermediate (>1 kDa) to large (>25 kDa) molecule kinetics.
AvCap The AvCap biosensor is a three-dimensional chemistry derived from the COOHV biosensor. It is modified with Biotin functional groups on a short spacer that allow for the selective irreversible capture of Avidin proteins. This chemistry is useful to capture conjugates that are prepared with Avidin proteins.