Pall ForteBio Protein Analysis Workshop & User Meeting

 

March 21, Airport Conference Center Frankfurt/Main

ForteBio


Date: Tuesday, March 21, 2017

Time: 8:30 - 17:00

Location: K 20 - Airport Conference Center; Airport Center 1, Airport Frankfurt/Main

Agenda now online! Network and knowledge-share with other Octet and BLItz system users, and learn more about how label-free assays are being used to characterize biomolecules in discovery and development. Furthermore, engage with Pall's Applications team for helpful tips and tricks during network breaks. The agenda includes informative talks by selected speakers who are experts in their field from both academia and industry:

  • Optimisation of Nanobody Interaction with MBP and the Influence of Maltose on the Binding Process
    Samira Gmür, FHNW Switzerland, School of Life Sciences
  • Rapid Identification of High Affinity Monoclonal Antibodies and Epitope Binning Using the ForteBio Octet RED384 System
    Dr. Andre ten Haaf, Bio-Rad
  • Application of ForteBio at BONMAB Platform in Development of Monoclonal Antibodies
    Alexey Misorin, BIOCAD
  • Live Cell-Based Bio-Layer Interferometry (cBLI)
    Thilo Riedel, Ph.D., Genmab

This year, new to the program will be a panel discussion on the current state and future direction of label-free analytical techniques.

Registration: The user meeting is free to attend, but reservations are required as seating is limited. Lunch is included.

 

Please Register

Agenda

8:30 - 9:30

Arrival and Registration

9:30 - 9:40

Welcome, Opening Remarks

9:40 - 10:10

Pall ForteBio Technology Overview and Update
Pall ForteBio

General overview of the latest developments in terms of instrumentation and applications.

10:10 - 10:40

Optimisation of Nanobody Interaction with MBP and the Influence of Maltose on the Binding Process
Samira Gmür, FHNW Switzerland, School of Life Sciences

A comparative binding study of selected Nanobodies and maltose-binding protein (MBP) using Octet, Isothermal Titration Calorimetry (ITC) and SPR was implemented to compare kinetic and thermodynamic properties.

10:40 - 11:00

Network Break Session

11:00 - 11:30

Rapid Identification of High Affinity Monoclonal Antibodies and Epitope Binning Using the ForteBio Octet RED384 System
Dr. Andre ten Haaf, Scientist Custom Antibodies, Bio-Rad

Development of an off-rate screening method of crude E. coli lysates containing monovalent Fab fragments obtained after phage display using the Octet RED384.

11:30 - 12:00

Application of ForteBio at BONMAB Platform in Development of Monoclonal Antibodies
Alexey Misorin, Scientist, BIOCAD

Introduction of a unique fully-automated Antibody Discovery Platform (BONMAB) for all stages of antigen-binding antibody fragments discovery.

12:00 - 13:30

Lunch

13:30 - 15:00

Panel Discussion – Current State, Future Directions of Label-free Techniques for Molecular Interaction Analysis
Moderated by Peter Spies, Hochschule für Life Sciences Basel

15:00 - 15:20

Network Break Session

15:20 - 16:05

Live Cell-Based Bio-Layer Interferometry (cBLI)
Thilo Riedel, Ph.D., Genmab

Monitoring binding interactions in real-time with live cells, creating a novel cell-based BLI, that has the potential to increase the versatility of label-free assays in screening labs for finding “hits” that rely on more complex signal transduction.

16:05 - 16:15

Closing Remarks

16:15 - 17:00

Networking