The determination of the affinity of a small molecule binding to a therapeutic target is a significant component of drug discovery and lead optimization. The Octet® RED and Octet HTX systems are designed with rapid data acquisition and sensitivity to enable the detection of small molecules and peptides in a simple, user-friendly workflow in conjunction with Super Streptavidin biosensors. Label-free analysis of the association and dissociation of a small molecule with the target protein of interest results in the determination of kinetic constants including the association rate constant (ka), dissociation rate constant (kd), and equilibrium dissociation constant (KD). View related articles.
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