For Quantitation and Kinetic Characterization of HIS-tagged Biomolecules
Taking advantage of the wide-spread use of poly-histidine protein tags (HIS-tag) in the bio-pharmaceutical industry, the Ni-NTA Biosensor further facilitates easy detection and rapid characterization of HIS-tagged molecules. Pre-immobilized with nickel-charged tris-nitriloacetic acid (Tris-NTA) from QIAGEN, the Ni-NTA Biosensor enables direct quantitation of HIS-tagged proteins and their easy capture for subsequent kinetic analyses with binding partners.
Ni-NTA (NTA) Biosensor datasheet.
- Direct and rapid quantitation of HIS-tagged biomolecules
- Easy capture of HIS-fusion proteins for kinetic analysis
- Designed for use in buffer and diluted complex media
- Compatible with Octet® and BLItz™ platforms
Figure 1: Kinetic analysis of the interaction between HIS-endostatin and an interaction partner anti-endostatin. 1X Kinetics Buffer was used as the matrix throughout and the assay temperature was 30°C. Data were processed and curve fit using a 1:1 binding model. Kinetic results for the interaction between HIS-endostatin and anti-endostatin using Ni-NTA biosensors are reported in the table.
For More Information
Visit our literature page to download technical notes, application notes, posters and customer presentations.