Sialic Acid (GlyS) Kit

 

High-throughput Glycan Screening of Crude and Purified Samples

  • No sample purification or digestion required; reduces preparation time by up to 3 hours
  • Screen for samples with desired sialylation levels – high, medium or low
  • Combine titer data with sialic acid data for more informed decisions
Example assay workflow for human IgG or human Fc-fusion proteins. Selective amplification of signal is from the protein of interest and not from host-cell protein (HCP).**

Figure 1: Example assay workflow for human IgG or human Fc-fusion proteins. Selective amplification of signal is from the protein of interest and not from host-cell protein (HCP). Please refer to the Sialic Acid (GlyS) Kit user guide for other assay workflows and additional materials required.

 

Glycosylation is considered among the most important post-translational modifications when developing new biologics. Having a significant impact on product performance and variability, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy. Protein glycosylation can affect isolation and purification steps (process consistency), pharmacokinetics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

ForteBio’s Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined with titer data using Octet Data Analysis HT software version 11.1 or higher to funnel through samples that are high producers and have desirable sialic acid content.

Assay Parameter Octet RED96e System, Sidekick and GlyS Kit Octet RED384 System, Sidekick and GlyS Kit Octet HTX System and GlyS Kit
Time to Results <2.5 hr/96 samples ˜1.5 hr/96 samples <1 hr/96 samples
Sample Type Crude cell culture supernatant Crude cell culture supernatant Crude cell culture supernatant

Table 1: Comparison of GlyS throughput on various Octet systems.

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