NEW Ni-NTA Biosensors for HIS-tagged Biomolecule Analysis
The robustness of the HIS tag and its ease of use have established it as one of the most commonly used tags in the biopharmaceutical industry. It’s commonly fused to recombinant proteins to expedite their capture, detection, and purification. This polyhistidine tag sequence exhibits strong binding to nickel (Ni2+).
Pre-immobilized with nickel-charged tris-nitrilotriacetic (Tris-NTA) groups, ForteBio’s new Ni-NTA Biosensor provides easy capture of HIS-fusion proteins for kinetic analysis (FIGURE 1, TABLE 1) and rapid quantitation of HIS-tagged biomolecules (FIGURE 2). The surface offers an excellent alternative to chemical protocols such as EDC/NHS and biotinylation.
This new biosensor is fully compatible with both our Octet® and BLItz™ platforms. When using the Ni-NTA Biosensor with BLItz, measurement of precious samples in volumes as low as 4 µL can be determined.
The Ni-NTA Biosensor offers researchers unmatched ease of use and time-to-result in a wide range of laboratory applications including quantitation of HIS fusion proteins, affinity characterization of interactions between HIS fusions and binding partners, efficient workflows for epitope binning/mapping and process optimization in development and quality control.
For more information, please contact your local ForteBio® representative or visit our NTA biosensor page to download the datasheet and technical notes.