The Octet® HTX System Raises the Bar on Performance

Dominic Andrada, Senior Product Manager, dominic_andrada@pall.com

HTX System

Pall ForteBio is pleased to announce the launch of the Octet HTX system, the first BLI instrument to monitor up to 96 biosensors simultaneously. The Octet HTX system provides the highest level of throughput, speed and assay flexibility for label-free protein quantitation and kinetic characterization. The new flagship of the Octet instrument family has the ability to read 8, 16, 32, 48 or 96 samples in parallel and provides unprecedented performance for all label-free assays.

Rapid whole plate detection is performed using the 96-biosensor mode, providing either quantitation of 96 samples in as little as 2 minutes or full-plate kinetic screening in minutes instead of hours. Use of the 32- or 48-biosensor modes enables large, complex assays such as epitope binning or multi-step quantitation to be completed in the shortest amount of time. The increased throughput and versatility of the Octet HTX system allows users to complete multiple label-free experiments within a single day, and obtain critical kinetic and quantitation data in a fraction of the time. Key applications that leverage the unique strengths of the Octet HTX system include epitope binning experiments, off-rate ranking, and full-plate titer determinations.

The Octet HTX system's multiple biosensor modes can be harnessed to significantly accelerate challenging applications such as small molecule binding kinetic characterization. For example, the 96-biosensor mode first can be used to rapidly immobilize an entire sensor tray. Next, the high-sensitivity 8- or 16-biosensor modes can be used to provide optimal data quality for characterization of small molecule binding interactions or for protein quantitation down to 50 ng/mL.

The Octet HTX system features advanced capabilities for epitope binning/cross-competition studies of monoclonal antibody panels. Analyses of pair-wise cross-blocking experiments are completed in record time, with a 16x16 matrix completed in 1 hour, or a 32x32 matrix in about 8 hours. Powerful epitope binning analysis software enables easy visualization of data sets and provides a flexible data matrix that accommodates a variety of cross-blocking formats (Figure 1).

Another target application for the Octet HTX system is primary screening in biotherapeutic drug discovery. Use of the 96-biosensor mode enables rapid label-free assays for titer determination, kinetic off-rate screening, or affinity screening of large clonal libraries. With the system's high throughput design, label-free screening data now can be generated much earlier in the discovery process. The coupling of laboratory automation with the Octet HTX system can provide even higher levels of throughput for label-free alternatives to ELISA and fluorescent assays.

Epitope binning

Figure 1: Epitope binning of 32x64 purified mAbs using the 32-biosensor mode, performed in 14 hours.