Octet® RED384 and System Specifications

Detection Technology Bio-Layer Interferometry (BLI)
Biosensor Type Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking
Information Provided
  • Kinetic and affinity analysis —kobs, ka, kd, KD
  • Kinetic screening for ka or kd
  • Binding specificity and cooperativity
  • Concentration monitoring of real-time binding (no need for background subtraction)
  • Automated concentration determinations
Data Presentation
  • Plots displaying kinetic binding, equation fits, and residuals of fits
  • Tabulated kinetic data and data charts
Sample Types Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Sample Plate Standard, 96-well and 384-well, black, flat-bottom microplate
Sample Volume
  • 80–130 µL/well (384-well plate) and 180–220 µL/well (96-well plate)
  • Nondestructive testing, easily recovered
Orbital Flow Capacity Static or 100–1500 rpm
Analysis Temperature Range (Ambient + 4 °C) – 40 °C, 1 °C increments)
Throughput Up to 16 assays in parallel; up to 96 assays per 96-well plate and 384 assays per 384-well plate
Analysis Time per Sample
  • hIgG quantitation in 2 minutes for 16 samples, ≤20 minutes for 96 samples and ≤75 minutes for 384 samples
  • Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
Baseline Noise
  • < 3 pm (RMS) for Octet RED384
  • < 6 pm (RMS) for Octet QK384
Quantitation Range
for hIgG
  • Octet RED384: 0.5 µg/mL–2000 µg/mL at 400 rpm, 0.05 µg/mL–100 µg/mL at 1000 rpm
  • Octet QK384: 1 µg/mL–700 µg/mL at 400 rpm, 0.1 µg/mL–100 µg/mL at 1000 rpm
Dimensions 30.1" H x 31.5" W x 31.5" D
(77 cm H x 80 cm W x 80 cm D)
Weight 150 lb (68.2 Kg)
Electrical Requirements Mains: AC 100–240 V, 5.0–2.0A, 50/60Hz, single phase
Power consumption: 195w (240 peak)
Safety Standards CE, CSA