Octet® RED96 System Specifications

SAMPLE AND ANALYSIS
Detection Technology Bio-Layer Interferometry (BLI)
Biosensor Type Disposable, single-use fiber optic biosensors with optional reuse by regeneration and/or re-racking
Information Provided
  • Kinetic and affinity analysis (kobs, ka, kd, KD)
  • Concentration monitoring (no need for background subtraction)
  • Automated concentration determinations
Data Presentation
  • Plots displaying kinetic binding, equation fits, and residuals of fits
  • Tabulated kinetic data and data charts
  • One-click concentration data analysis including generation of calibration curves and output of tabulated concentration data
Sample Types Proteins, antibodies, peptides, media containing serum, buffers containing DMSO, periplasmic fractions, untreated cell culture supernatants, and crude cell lysates
Sample Plate Standard, 96-well, black, flat-bottom microplate
Sample Volume
  • 180–220 µL/well
  • Nondestructive testing
Orbital Flow Capacity Static or 100–1500 rpm
Analysis Temperature Range (Ambient + 4°C) – 40°C, 1°C increments
QUANTITATION AND KINETICS
Throughput Up to 8 assays in parallel; up to 96 assays per 96-well plate
Analysis Time per Sample
  • hIgG quantitation in 2 minutes for 8 samples, ≤32 minutes for 96 samples
  • Real-time kinetic binding experiments from 5 minutes to 4 hours for kinetic analysis
Quantitation Range for hIgG 0.5 µg/mL to 2000 µg/mL at 200 rpm,
0.05 µg/mL to 100 µg/mL at 1000 rpm
Baseline Noise ≤3 pm (RMS)
PHYSICAL SPECIFICATIONS
Dimensions 18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)
Weight 63 lb (28.6 Kg)
Data Handling and Storage
  • Mains: AC 100–240 V, 5.0–2.0 A, 50/60 Hz, single phase
  • Power consumption: 120W (240 peak)
Safety Standards CE and CSA