Amine Reactive Second-Generation (AR2G) Biosensors

EDC/Sulfo-NHS Based Immobilization for Kinetic Characterization of Primary Amine Containing Biomolecules

  • Rapid immobilization of proteins and peptides
  • Rapid assay development
  • Second-generation amine reactive detection surface (AR2G)

Amine Reactive Second-Generation (AR2G) Biosensors

The AR2G biosensor is a newly engineered biocompatible surface matrix that delivers enhanced performance for kinetic assays compared to the original Amine Reactive (AR) biosensor for most proteins (Figure 1). AR2G routinely achieves greater signal intensity than the AR biosensor during both the loading (immobilization of ligand) and association (detection of analyte) steps of an assay while delivering the same reliable kinetic values. Evaluation of four antibody proteins demonstrated an increase in the intensity of the loading signal by an average of 4-fold with one example extending to 7.5-fold (Figure 1). A screen of several buffer systems determined that the AR2G chemistry is highly compatible with acetate buffer, which is both commonly available and cost-effective. A decrease in non-specific binding events to the AR2G surface compared to AR has also been demonstrated.

Resources of Amine Reactive Second-Generation (AR2G) Biosensors

Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

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Data Sheet Amine Reactive Second-Generation Biosensors

The Dip and Read™ Amine Reactive 2nd-Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Product Insert Amine Reactive Biosensors

The Amine Reactive Biosensors enable the coupling of proteins to carboxylate groups on the biosensor surface via accessible amine groups. The coupling procedure is a simple three step protocol based on well characterized amide bond formation chemistry. Amine coupling can be fully automated online or can be batch immobilized offline.

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Scientific Poster Streamlining assay development with Amine Reactive Biosensors on the Octet System for kinetic analysis of therapeutics

Streamlining assay development with Amine Reactive Biosensors on the Octet System for kinetic analysis of therapeutics

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Technical Guide Dip and Read™ Amine Reactive Second-Generation (AR2G) Reagent Kit

The Amine Reactive 2nd Generation (AR2G) Reagent Kit is intended for use with the AR2G biosensor to enable covalent immobilization of a protein, peptide or other primary amine containing biomolecule onto the AR2G biosensor surface via a stable amide bond. Immobilization of proteins is achieved through standard EDC-catalyzed amide bond formation to create a covalent bond between a reactive amine on the protein and the carboxy-terminated biosensor surface. The kit contains sufficient reagents to perform 1000 standard immobilization reactions.

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Technical Guide Dip and Read™ Amine Reactive Second-Generation (AR2G) Biosensors

Amine Reactive 2nd Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes.

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Product Part Number
Biosensor / Amine Reactive 2nd Generation (AR2G) Case #18-5094
Biosensor / Amine Reactive 2nd Generation (AR2G) Pack #18-5093
Biosensor / Amine Reactive 2nd Generation (AR2G) Tray #18-5092

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