Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors

For Label-Free Detection, Quantitation, and Kinetic Analysis of Human Fab and IgG

  • Perform kinetic screening, off-rate ranking, and epitope binning of Fabs in crude or partially purified samples
  • Easy capture of human Fab or IgG for kinetic analysis of binding to target antigen or Fc receptors (Kon, Koff, KD)
  • Rapid quantitation of all subclasses of human Fab, F(ab′)2 and IgG
  • No recognition towards human kappa or lambda light chains
  • Improved Fab capture affinity, baseline stability, and regeneration over original FAB biosensors

Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors

Anti-Human Fab-CH1 2nd Generation (FAB2G) biosensors come pre-immobilized with a high-affinity ligand that is specific for the CH1 region of Human IgG. In conjunction with the Octet® and BLItz® Systems, FAB2G biosensors provide a rapid and label-free solution for analysis of Fab fragments or full-length human IgG for lead identification and optimization, epitope binning, cell line screening, process development, and QC applications. The anti-human CH1 ligand has no recognition towards free light chains or cross-reactivity with murine or bovine IgG. This high level of specificity enables direct analysis of IgG or Fab analytes directly from complex samples such as cell culture supernatants, lysates, or partially purified samples, offering a time-saving alternative to traditional analytical methods.

Resources of Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors

Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

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Application Note Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform

Fc gamma receptors (FcγRs) are membrane glycoproteins with affinity for the Fc region of immunoglobulin G (IgG). FcγRs expressed on the surface of immune effector cells play a key role in initiating Fc effector functions such as antibody-mediated cell-dependent cytotoxicity (ADCC)1, which is a major mechanism of action of therapeutic monoclonal antibodies

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Application Note Analysis of FcRn-Antibody Interactions on the Octet Platform

The Fc region of human IgG contributes to a number of beneficial biological and pharmacological characteristics of therapeutic antibodies.

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Data Sheet Anti-Human Fab-CH1 2nd Generation Biosensors

Human antibodies are the most vital research candidates in drug discovery and development of bio-therapeutics. The detection and characterization of human IgG is of paramount importance for research scientists.

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Data Sheet Dip and Read Anti-Human Fab-CH1 Biosensors

Human antibodies are the most vital research candidates in drug discovery and the development of bio-therapeutics. The detection and characterization of human IgG is of paramount importance for research scientists. The Anti-Human Fab-CH1 Biosensor is pre-immobilized with a llama antibody fragment (BAC BV ) that binds specifically to the CH1 domain of all four human IgG subclasses (Figure 1), and enables an easy and rapid method of quantitation (Figure 2) and kinetic characterization (Figure 3) of human Fab, F(ab’)2, and IgG.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Technical Guide Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays

The Anti-Human Fab-CH1 Biosensor consists of a high affinity antihuman CH1 affinity ligand pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the AntiHuman Fab-CH1 Biosensor provides a rapid and label-free method for Human Fab/F(ab’)2/IgG quantitation and kinetic analysis. The high specificity of the antibody fragment-based biosensor enables direct analysis of human Fab/F(ab’)2/IgG analytes in crude lysates, column eluents, cell lysates and cell culture supernatants, serving as a time-saving alternative to traditional analytical methods. For more information on kinetic analyses using the Anti-Human FabCH1 Biosensor, please see ForteBio Technical Note 34, Anti-Human Fab-CH1 Biosensor Kinetics Assays.

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Technical Guide Anti-Human Fab-CH1 Biosensor Kinetics Assays

The Anti-Human Fab-CH1 Biosensor consists of a high-specificity anti-Human Fab-CH1 antibody fragment pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the Anti-Human Fab-CH1 Biosensor provides a rapid and label-free method for Human F(ab’)2/Fab/IgG quantitation and kinetic analysis. The high specificity of the antibody fragment-based biosensor enables direct analysis of Human F(ab’)2/Fab/IgG analytes in crude lysates, column eluents, cell lysates and cell culture supernatants, serving as a time-saving alternative to traditional analytical methods. For more information on quantitation analyses using the AntiHuman Fab-CH1 Biosensor, please see ForteBio Technical Note 33, Anti-Human Fab-CH1 Biosensor Quantitation Assays.

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Product Part Number
Biosensor / Anti-Human Fab-CH1 2nd Generation (FAB2G) Case #18-5127
Biosensor / Anti-Human Fab-CH1 2nd Generation (FAB2G) Pack #18-5126
Biosensor / Anti-Human Fab-CH1 2nd Generation (FAB2G) Tray #18-5125

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