Anti-Mouse IgG Fc Capture (AMC) Biosensors

For Immobilization and Subsequent Kinetic Characterization of Mouse IgGs and other Fc-Containing Proteins

  • Complete kinetic analysis (kon, kdis and KD) between mouse Fc-containing proteins and target analytes
  • Off-rate ranking of hybridoma and stable cell-line supernatants
  • Epitope binning/mapping (from crude or purified samples)

Anti-Mouse IgG Fc Capture (AMC) Biosensors

Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes. Immobilization of mouse Fc-containing proteins is achieved through a factory immobilized anti-mouse Fc-specific antibody whose high-affinity for the mouse Fc domain provides the stable baseline required for demanding kinetics applications. AMC biosensors can be regenerated up to 10 times via a standard low-pH protocol in as little as two minutes for select applications.

Resources of Anti-Mouse IgG Fc Capture (AMC) Biosensors

Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

Read more

Data Sheet Anti-Mouse IgG Fc Capture (AMC) Biosensors

Dip and Read™ Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes.

Read more

Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

Read more

Technical Guide Dip and Read™ Anti-Mouse IgG Fc Capture Biosensors

Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes (Figure 1). Immobilization of mouse Fc-containing proteins is achieved through a factory immobilized anti-mouse Fc-specific antibody whose high affinity for the mouse Fc domain provides the stable baseline required for demanding kinetics applications. Cost-effective regeneration of the biosensors and the ability to directly immobilize mouse Fc-containing proteins from crude matrices make AMC biosensors extremely useful in high-throughput applications.

Read more

Technical Guide Human and Mouse IgG Subtype Identification Using the Octet™ Platform

This technical note describes a rapid assay method for quantitation and subtype identification of human and mouse IgG. The antibodies can be quantified in buffer, serum-free media or lysates, using the ForteBio® Anti-Human IgG Fc or Anti-Murine Fv biosensors. Using the same biosensors, the subtype of the antibody can be identified using subtype-specific secondary antibodies. The flexible microplate-based format of the Octet platform allows up to 48 samples to be analyzed on an Octet QK or Octet RED system and up to 96 samples on an Octet QK384 or Octet RED384 system in 1 hour.

Read more

White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

Read more
Product Part Number
Biosensor / Anti-Mouse Fc Capture (AMC) Case #18-5090
Biosensor / Anti-Mouse Fc Capture (AMC) Pack #18-5089
Biosensor / Anti-Mouse Fc Capture (AMC) Tray #18-5088

We use cookies to personalize content and improve your browsing experience.

We also use analytics & advertising services. To opt-out click for more information.