Anti-Penta-HIS (HIS1K) Biosensors

For Label-Free Quantitation and Kinetic Analysis of His-Tagged Recombinant Proteins

  • High specificity and affinity to a six-histidine or penta-histidine peptide tag (His-tag)
  • Designed for use in buffer, complex media, column eluates, and cell lysates
  • Binds His-tagged proteins even in the presence of imidazole
  • Direct capture of His-tagged proteins for kinetic analysis with interacting protein analyte
  • Easy to use, label-free, no wash protocol

Anti-Penta-HIS (HIS1K) Biosensors

The polyhistidine protein tag (His-tag), commonly engineered into proteins during cloning, can be used to facilitate recombinant protein purification and detection by exploiting the chelating properties of histidine to an immobilized metal. The Anti-Penta-HIS (HIS1K) Biosensor consists of high affinity, high specificity Penta-His antibody from Qiagen pre-immobilized on a fiber optic biosensor. In conjunction with the Octet® and BLItz® systems, the HIS1K Biosensor provides a rapid and label-free method for His-tagged protein quantitation and kinetic characterization. The high specificity of the antibody-based biosensor enables the direct capture and quantitation of His-tagged proteins even in crude cell lysates or supernatants.

Resources of Anti-Penta-HIS (HIS1K) Biosensors

Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

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Data Sheet Anti-Penta-HIS (HIS1K) Biosensors

The polyhistidine tag, commonly known as His-tag, is fused to recombinant proteins as a means of facilitating detection and purification. The Dip and Read™ Anti-Penta-HIS (HIS1K) Biosensor consists of high affinity, high specificity Penta-His antibody from QIAGEN pre-immobilized on a ForteBio fiber optic biosensor.

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Data Sheet Anti-Penta-HIS (HIS) Biosensors

The six-histidine or penta-histidine peptide tag (HIS-tag) is a common peptide tag fused to recombinant proteins during cloning. Many tools have been developed enabling the tag to be used for easy detection and purification of tagged proteins. The AntiPenta-HIS biosensor, for use on the Octet system, now provides a rapid, label-free method for quantification of HIS-tagged proteins. This biosensor comes with the highly specific Qiagen Penta-HIS antibody pre-immobilized on the surface and is ready to use for specific detection and quantitation of HIS-tagged proteins.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Technical Guide Anti-Penta-HIS (HIS1K) Biosensors for Label-free Analysis of His-tagged Proteins

The polyhistidine-tag (His-tag) is a common peptide tag added to recombinant proteins during cloning. Many tools have been developed that enable this tag to be used for detection and purification of tagged proteins. Read more
Product Part Number
Biosensor / Anti-Penta-HIS (HIS1K) Case #18-5122
Biosensor / Anti-Penta-HIS (HIS1K) Pack #18-5121
Biosensor / Anti-Penta-HIS (HIS1K) Tray #18-5120

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