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High Precision Streptavidin (SAX) Biosensors

For High Precision Quantitation and Kinetic Analysis

  • Rapid and stable capture of biotinylated molecules
  • High precision quantitation and kinetic analyses
  • QC-tested to meet precision-controlled coefficient of variation (CV) specification of <4%

High Precision Streptavidin (SAX) Biosensors

The new High Precision Streptavidin Biosensor (SAX) was specifically developed and qualified for applications in drug discovery and quality control labs that have stringent requirements for assay precision and robustness in quantitation and binding kinetics characterization assays. For quantitation assays involving non-Ab protein analyte, biotinylated capture molecules loaded on SAX biosensors may be used to generate data with high precision. When running assays for effector function analysis, antibody engineering, quality control studies or wherever else binding kinetics characterization is performed, the best assay performance can be attained using SAX biosensors. SAX biosensors are tested for CV <4% at ForteBio. The actual performance in customer's assays may vary depending up on the choice of sample matrix, ligand and analyte, and other assay conditions.

Resources of High Precision Streptavidin (SAX) Biosensors

Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Customized quantitation of recombinant therapeutic proteins using High Precision Streptavidin biosensors (SAX)

The accurate determination of recombinant protein titer is critical to the selection of high-producing clones during cell line development and in optimization of bioreactor conditions during production of therapeutic proteins.

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Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

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Data Sheet High Precision Streptavidin (SAX) Biosensors

Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors enable quick and easy modification and customization of the biosensor with any biotin-tagged molecule for quantitative and kinetic measurements.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Technical Guide High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic Assays

Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors enable quick and easy modification and customization of the biosensor with any biotintagged molecule for quantitative and kinetic measurements.

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Technical Guide Biotinylation of Protein for Immobilization onto Streptavidin Biosensors

The interaction between streptavidin and biotin is widely used as a system for the rapid, stable and irreversible non-covalent binding of biological molecules. The Octet® platform’s Streptavidin biosensors have been developed for the immobilization of biotinylated ligands for both quantitation and kinetic applications. The first protein immobilized onto the Streptavidin biosensors must be biotinylated prior to assaying on the Octet system.

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Technical Guide Regeneration Strategies for Streptavidin Biosensors on the Octet Platform

The ForteBio Octet platform offers powerful tools for the real-time, label-free analysis of protein-protein interactions. Together, the Octet systems and Streptavidin (SA) biosensors provide a flexible format for kinetic analysis and kinetic screening for any biological interaction in which one molecule can be biotinylated. Read more

Technical Guide Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors

ForteBio Streptavidin biosensors enable the immobilization of a biotinylated ligand onto the biosensor surface. The immobilized molecule can then be used in subsequent kinetic or custom quantitation applications.

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White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

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