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Ni-NTA (NTA) Biosensors

For Quantitation and Kinetic Characterization of HIS-tagged Biomolecules

  • Direct and rapid quantitation of HIS-tagged biomolecules
  • Easy capture of HIS-fusion proteins for kinetic analysis
  • Designed for use in buffer and diluted complex media
  • Compatible with Octet® and BLItz™ platforms

Ni-NTA (NTA) Biosensors

Taking advantage of the wide-spread use of poly-histidine protein tags (HIS-tag) in the bio-pharmaceutical industry, the Ni-NTA Biosensor further facilitates easy detection and rapid characterization of HIS-tagged molecules. Pre-immobilized with nickel-charged tris-nitriloacetic acid (Tris-NTA) from QIAGEN, the Ni-NTA Biosensor enables direct quantitation of HIS-tagged proteins and their easy capture for subsequent kinetic analyses with binding partners.

Resources of Ni-NTA (NTA) Biosensors

Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform

Fc gamma receptors (FcγRs) are membrane glycoproteins with affinity for the Fc region of immunoglobulin G (IgG). FcγRs expressed on the surface of immune effector cells play a key role in initiating Fc effector functions such as antibody-mediated cell-dependent cytotoxicity (ADCC)1, which is a major mechanism of action of therapeutic monoclonal antibodies

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Application Note Octet Potency Assay: Development, Qualification and Validation Strategies

Kinetic analysis of biomolecular interactions is critical during drug discovery and development. The affinity of an interaction directly affects the dose required for a biopharmaceutical to be effective.

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Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

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Data Sheet Ni-NTA Biosensors

The penta- or six-histidine peptide tag (HIS) is commonly fused to recombinant proteins to expedite capture, detection, and purification.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Technical Guide Ni-NTA Biosensor Quantitation Assays

A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purification.

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Technical Guide Ni-NTA Biosensor Kinetic Assays

A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purification. 

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White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

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