Protein A (ProA) Biosensors

For Determination of Antibody Concentration

  • Direct measurement of immunoglobulins (IgG)
  • Assay samples without centrifugation
  • Fast turnaround of results
  • Broad dynamic range
  • Correlates to HPLC
  • Direct measurement of immunoglobulins (IgG) 25ng – 2mg/mL

Protein A (ProA) Biosensors

ForteBio's Protein A biosensors enable rapid antibody quantitation from crude lysates, cell culture supernatants and complex matrices. The direct quantitation assay format of the Protein A biosensor provides ELISA data in less than 2 minutes and can be automated for high throughput screening efforts. Using an established pH-based regeneration protocol, the biosensors can be regenerated multiple times to provide a cost-effective and time-saving assay format.

Resources of Protein A (ProA) Biosensors

Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

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Application Note Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics

Analytical techniques that measure protein quantity and quality are used in nearly all stages of research, process development and manufacturing of biotherapeutics. UV spectroscopy, ELISA and HPLC have been in use for decades for protein quantitation in physiologi- cal and process samples, and continue to be the workhorses despite their many limitations.

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Application Note A Fast and High Precision Influenza Vaccine Potency Assay

Vaccines are biological preparations that contain agents resembling disease causing microorganisms, and can improve immunity against a specific disease. They are typically prepared from inactivated or weakened forms of the microbe or its toxins, or surface proteins.

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Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

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Application Note CaptureSelect affinity ligands for antibody detection and characterization

BAC’s CaptureSelect affinity ligands provide a unique group of molecules that can be designed for almost any purification target, with as narrow or as broad specificity as is required. The fragments are derived from the unique heavy chain antibodies found in Camelidae. Heavy chain antibodies are devoid of the entire light chain and CH1 domain found in conventional antibodies (Figure 1). Without the light chain, antigens are bound only by the variable domain of the heavy chain (VHH), without a loss in binding affinities compared to conventional antibodies.

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Application Note Rapid, reliable quantitation of Fc-Fusion protein in cell culture supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein (Protein 1) in crude cell culture supernatants. The group had historically used HPLC for protein quantitation during screening and selection of promising mammalian clones at every scale-up step, from 96- well microplates to 3L-bioreactors.

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Data Sheet Protein A Biosensors

Accurate antibody quantitation is critical to selecting cell lines for developing and optimizing antibody production. Traditional methods for measuring antibody concentration include HPLC, ELISA and densitometry—all of which have long analysis times, lack of specificity, and precision.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Product Insert Protein A Biosensors

Protein A is a cell wall protein derived from Staphylococcus aureus that exhibits unique binding properties for immunoglobulins in a variety of mammalian species. In particular, Protein A binds with the Fc region of IgG. A variety of methods for purification and analysis based on Protein A are widely used both in the development and production of antibody and protein therapeutics.

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Scientific Poster Rapid Determination of Dynamic Binding Capacity of Resins using Biolayer Interferometry (Octet)

Determination the dynamic binding capacity of resins is an essential step in optimization of a purification process.

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Scientific Poster Developing label-free, real-time detection of human polyclonal and monoclonal IgG with a Protein A biosensor on ForteBio's Octet

Developing lebel-free, real-time detection of human polyclonal and monoclonal IgG with a Protein A biosensor on ForteBio's Octet

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Scientific Poster Influenza vaccine titre determination using Biolayer Interferometry(BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly scaling each process step. Traditionally Single Radial Immunodiffusion (SRID) assays have been used as the ‘gold standard’ but the assay requires very skilled operators to obtain reproducible results and is relatively low throughput. ELISAs have also been used to determine titre but have lower precision and dynamic range. 

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Scientific Poster High throughput Bio-Layer Interferometry in Therapeutic Antibody Discovery and Development

Bio-Layer Interferometry (BLI) plays an important role in the antibody discovery and development processes at Genmab. Integrating the quantification, kinetic and affinity output of our ForteBio Octet BLI platform with the data analysis capabilities of ActivityBase XE enables us to perform “high value” screening within early antibody discovery.

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Technical Guide Residual Protein A Detection Kit

The Residual Protein A Detection Kit is intended for the detection and quantitation of recombinant Protein A or other Protein A constructs such as MabSelect SuRe TM . It has been developed as a simpler, faster alternative to ELISA method with reduced hands-on time for customers who require a sensitive and robust assay for measuring small amounts of leached Protein A in an- tibody or Fc-fusion protein samples

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White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

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Product Part Number
Biosensor / Protein A (ProA) Case #18-5013
Biosensor / Protein A (ProA) Pack #18-5012
Biosensor / Protein A (ProA) Tray #18-5010

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