Protein G (ProG) Biosensors

For Determination of Antibody Concentration:

  • Direct detection of human, mouse and rat IgGs
  • Regenerable and cost-effective format
  • Obtain ELISA data in minutes

Protein G (ProG) Biosensors

ForteBio's Protein G biosensors provide a rapid and direct method for quantifying mammalian IgGs from buffer, media or other complex matrices. Protein G is pre-immobilized onto the biosensors, and binds to rodent and many other mammalian IgGs with higher affinity than Protein A, but does not bind to IgM, IgD or IgA. In combination with the Octet® system, Protein G biosensors can streamline bioprocessing applications by providing precise results with minimal sample handling and turnaround times as fast as two minutes. The biosensors can be regenerated multiple times, providing a cost-effective and time-saving assay format. Using Protein G Biosensors, the Octet System supports applications from hybridoma screening to media development and chromatography mass balance.

Resources of Protein G (ProG) Biosensors

Application Note CaptureSelect affinity ligands for antibody detection and characterization

BAC’s CaptureSelect affinity ligands provide a unique group of molecules that can be designed for almost any purification target, with as narrow or as broad specificity as is required. The fragments are derived from the unique heavy chain antibodies found in Camelidae. Heavy chain antibodies are devoid of the entire light chain and CH1 domain found in conventional antibodies (Figure 1). Without the light chain, antigens are bound only by the variable domain of the heavy chain (VHH), without a loss in binding affinities compared to conventional antibodies.

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Application Note Rapid, reliable quantitation of Fc-Fusion protein in cell culture supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein (Protein 1) in crude cell culture supernatants. The group had historically used HPLC for protein quantitation during screening and selection of promising mammalian clones at every scale-up step, from 96- well microplates to 3L-bioreactors.

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Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Biomolecular Binding Kinetics Assays on the Octet Platform

Direct measurement of biomolecular interactions plays an important role in biotherapeutic drug discovery and development. Label-Free analytical technologies such as the Octet® platform from Pall ForteBio provide a powerful means to obtain accurate information about rate of biomolecular complex formation and complex stability, key components of a drug-target interaction.

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Application Note A Fast and High Precision Influenza Vaccine Potency Assay

Vaccines are biological preparations that contain agents resembling disease causing microorganisms, and can improve immunity against a specific disease. They are typically prepared from inactivated or weakened forms of the microbe or its toxins, or surface proteins.

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Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

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Data Sheet Protein G Biosensors

Protein G biosensors provide a rapid and direct method for quantification of many types of mammalian IgG from buffer, media or other complex matrices.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Scientific Poster Influenza vaccine titre determination using Biolayer Interferometry(BLI)

Fast, accurate determination of vaccine titre during influenza vaccine manufacture is important in understanding process performance and correctly scaling each process step. Traditionally Single Radial Immunodiffusion (SRID) assays have been used as the ‘gold standard’ but the assay requires very skilled operators to obtain reproducible results and is relatively low throughput. ELISAs have also been used to determine titre but have lower precision and dynamic range. 

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Technical Guide Protein G Biosensors

Protein G biosensors provide a rapid and direct method for quantifying mammalian IgGs from buffer, media or other complex matrices.

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Product Part Number
Biosensor / Protein G (ProG) Case #18-5084
Biosensor / Protein G (ProG) Pack #18-5083
Biosensor / Protein G (ProG) Tray #18-5082

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