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Protein L (ProL) Biosensors

For Whole Molecule Antibody and Antibody Fragment Quantitation

  • Broader recognition of Ig isotypes and species than Protein A or G
  • Regenerable and cost-effective format
  • Binds whole molecule Ab and FAb fragments
  • Quantitate in crude matrices

Protein L (ProL) Biosensors

Protein L biosensors provide a rapid and direct method for quantitating a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb fragments and single chain variable fragments, from buffer, conditioned media or complex matrices. The recombinant Protein L, which is factory-immobilized onto the biosensor, recognizes a wider range of antibody classes than both Protein A and Protein G (Table 1), including IgG, IgM, IgA, IgE and IgD. The Protein L biosensor is especially useful for quantifying antibodies and antibody fragments from serum based culture because Protein L does not bind bovine immunoglobulins, which often contaminate serum supplements. Note that Protein L does not recognize all kappa light chains. For example, Protein L recognizes mouse VkI (but not VkII, VkIII and VkIV) and human VkI, VkIII and VkIV (but not VkII).

Resources of Protein L (ProL) Biosensors

Application Note CaptureSelect affinity ligands for antibody detection and characterization

BAC’s CaptureSelect affinity ligands provide a unique group of molecules that can be designed for almost any purification target, with as narrow or as broad specificity as is required. The fragments are derived from the unique heavy chain antibodies found in Camelidae. Heavy chain antibodies are devoid of the entire light chain and CH1 domain found in conventional antibodies (Figure 1). Without the light chain, antigens are bound only by the variable domain of the heavy chain (VHH), without a loss in binding affinities compared to conventional antibodies.

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Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

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Data Sheet Protein L Biosensors

Dip and Read™ Protein L (ProL) biosensors provide a rapid and direct method for quantifying a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb fragments and single chain variable fragments, in buffer, conditioned media or complex matrices.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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