Ready BLI Detection Kit – Residual Protein A
The Ready BLI Detection Kit - Residual Protein A (RPA) applies the principles of BLI to enable sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization, or centrifugation steps.
Dip and Read™ RPA biosensors with pre-immobilized chicken anti-Protein A antibody are sequentially dipped into treated samples, buffers, and detection antibody arrayed in 96-well microplates to create a microscale immunoassay on the biosensor tip. Signal detection occurs in real time at the secondary antibody step without need for conjugates or enzymatic reactions.