Residual Protein A Detection Kit

For Quantitation of Leached Protein A in Bioprocess Samples

  • Quantitation of recombinant Protein A constructs and MabSelect SuRe™
  • Sensitivity down to 100 pg/mL
  • Automated detection with minimal hands-on time
  • Analyze 96 samples in under 2 hours on the Octet HTX system

Ready BLI Detection Kit – Residual Protein A

The Ready BLI Detection Kit - Residual Protein A (RPA) applies the principles of BLI to enable sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization, or centrifugation steps.

Dip and Read™ RPA biosensors with pre-immobilized chicken anti-Protein A antibody are sequentially dipped into treated samples, buffers, and detection antibody arrayed in 96-well microplates to create a microscale immunoassay on the biosensor tip. Signal detection occurs in real time at the secondary antibody step without need for conjugates or enzymatic reactions.

Resources of Residual Protein A Detection Kit

Data Sheet Ready BLI Detection Kit – Residual Protein A

Detecting and minimizing Protein A contamination is a critical quality control step in antibody therapeutic development and production. The Ready BLI Detection kit - Residual Protein A from Pall ForteBio applies the principles of BLI to enable sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization, or centrifugation steps.

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Scientific Poster Quantitation of Residual Protein A and MabSelect SuRe using a “Dip and Read” Assay

Detection of residual Protein A in product purified using a Protein A, or similar column matrix such as MabSelect SuRe™, is a critical quality control step in antibody therapeutic development and production. During the IgG purification process, Protein A can leach from the purification column and co‐elute with the IgG. This contaminant in the therapeutic mixture can cause adverse reactions in patients and thus must be detected and minimized. The Octet family of instruments utilizes a unique label‐free “dip and read” detection technology to quantify biomolecules in solution. ForteBio has developed a new kit for use on this system to detect and quantify residual Protein A. The new Dip and Read™ Residual Protein A Detection Kit contains biosensors and all the reagents required to quantitate Protein A and MabSelect SuRe. The data presented here demonstrates that the Octet system can accurately and precisely detect 100 pg/mL Protein A or MabSelect SuRe in bioprocess samples.

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Technical Guide Ready BLI Detection Kit – Residual Protein A

The Ready BLI Detection kit for Residual Protein A is intended for the detection and quantitation of recombinant Protein A or other Protein A constructs such as MabSelect SuRe™.

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Product Part Number
Residual Protein A Detection Kit #18-5128

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