Sialic Acid (GlyS) Kit

High throughput Glycan Screening of Crude and Purified Samples

  • No sample purification or digestion required; reduces preparation time by up to 3 hours
  • Screen for samples with desired sialylation levels – high, medium or low
  • Combine titer data with sialic acid data for more informed decisions

High throughput Glycan Screening of Crude and Purified Samples

 

glys

 

Figure 1: Example assay workflow for human IgG or human Fc-fusion proteins. Selective amplification of signal is from the protein of interest and not from host-cell protein (HCP).

Glycosylation is considered among the most important post-translational modifications when developing new biologics. Having a significant impact on product performance and variability, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy. Protein glycosylation can affect isolation and purification steps (process consistency), pharmacokinetics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

ForteBio’s Sialic Acid (GlyS) Kit provides a rapid and convenient method for relative screening of terminal sialic acid content in crude or purified samples. Sialic acid data can be combined with titer data using Octet Data Analysis HT software version 11.1 or higher to funnel through samples that are high producers and have desirable sialic acid content.

Assay Parameter Octet RED96e System, Sidekick and GlyS Kit Octet RED384 System, Sidekick and GlyS Kit Octet HTX System and GlyS Kit
Time to Results <2.5 hr/96 samples ˜1.5 hr/96 samples <1 hr/96 samples
Sample Type Crude cell culture supernatant Crude cell culture supernatant Crude cell culture supernatant

Table 1: Comparison of GlyS throughput on various Octet systems.

Resources of Sialic Acid (GlyS) Kit

Data Sheet Sialic Acid (GlyS) Kit

Glycosylation is considered among the most important post-translational modifications when developing new biologics. Having a significant impact on product performance and variabil- ity, glycosylation is a critical quality attribute (CQA) influencing product safety and efficacy. Protein glycosylation can affect iso- lation and purification steps (process consistency), pharmacoki- netics (half-life) properties and in vitro stability (product shelf-life). Sialic acid content is especially important as it can impact the stability and clearance of a protein.

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Flyer Cell line development: accelerating antibody discovery by monitoring titer and glycosylation with the Octet platform

Cell line development typically includes the screening of thousands of clones in an effort to find the few that are stable, grow as expected, and produce high yields of the bioproduct. The time it takes from engineering an optimal cell line to the production of the target biologic can be prohibitive and may differ from molecule to molecule.

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Scientific Poster High throughput sialylation screening on Octet label free-instrument for expediting clone selection process in cell line development

Explore more about the high-throughput method for relative screening of terminal sialic acid content was developed on the Octet platform to expedite cell line development.

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Technical Guide Sialic Acid (GlyS) Kit

The Sialic Acid (GlyS) Kit is intended for relative screening of terminal sialic acid in crude or purified samples. It has been developed as a simpler and faster complement to HPLC/MS/LCMS methods with reduced hands-on time for customers who desire more information earlier in their workflow.

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Product Part Number
Sialic Acid (GlyS) Kit #18-5135

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