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Streptavidin (SA) Biosensors

For Kinetic Analysis, Screening, and Quantitation of Most Proteins

  • Immobilization of biotinylated proteins for kinetic analysis of biomolecular interaction pairs
  • Immobilization of biotinylated proteins for quantitative analysis of proteins and their target analyte

Streptavidin (SA) Biosensors

ForteBio's Streptavidin (SA) biosensors provide robust access to highly sensitive custom biosensors through exploitation of the high affinity streptavidin-biotin interaction. Biological molecules, including proteins and nucleic acids, can be biotinylated using established protocols and immobilized onto the Streptavidin biosensor surface for custom assay development. The high sensitivity and stable baseline of the Streptavidin biosensor supports kinetic as well as quantitation experiments.

Resources of Streptavidin (SA) Biosensors

Application Note Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular interactions critical to mapping the interactome as well as drug discovery efforts. Due to their integration into a lipid bilayer, in vitro characterization of molecular interactions of membrane proteins presents a unique challenge compared to soluble proteins.Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular interactions critical to mapping the interactome as well as drug discovery efforts. Due to their integration into a lipid bilayer, in vitro characterization of molecular interactions of membrane proteins presents a unique challenge compared to soluble proteins.

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Application Note Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control

The group was able to develop a working Fab activity assay on the Octet RED system in less than a week. Relative to the overnight incubation and four-hour assay time of their ELISA protocol, the Octet assay provided an analysis time of only one hour per 96-well microplate, including sample preparation time. This Octet assay was used to monitor Fab activity for all process development studies.

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Application Note Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform

Fc gamma receptors (FcγRs) are membrane glycoproteins with affinity for the Fc region of immunoglobulin G (IgG). FcγRs expressed on the surface of immune effector cells play a key role in initiating Fc effector functions such as antibody-mediated cell-dependent cytotoxicity (ADCC)1, which is a major mechanism of action of therapeutic monoclonal antibodies

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Application Note Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics

Analytical techniques that measure protein quantity and quality are used in nearly all stages of research, process development and manufacturing of biotherapeutics. UV spectroscopy, ELISA and HPLC have been in use for decades for protein quantitation in physiologi- cal and process samples, and continue to be the workhorses despite their many limitations.

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Data Sheet Streptavidin (SA) Biosensors

ForteBio Streptavidin (SA) biosensors are designed for immobilization of biotin labeled proteins for use in assaying protein:protein interactions using the Octet® platform. The systems support applications for kinetics characterization and quantitation of analytes binding to the immobilized protein.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Scientific Poster Detection of STEC Strains O157:H7, O26 and O111 using Biosensor System

Escherichia coli O157:H7, a strain of enterohemorrhagic, Shiga toxin-producing E. coli (STEC), causes gastroenteritis, resulting in (bloody) diarrhea and sometimes acute kidney failure due to HUS (hemolyticuremic syndrome).

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Scientific Poster High throughput Bio-Layer Interferometry in Therapeutic Antibody Discovery and Development

Bio-Layer Interferometry (BLI) plays an important role in the antibody discovery and development processes at Genmab. Integrating the quantification, kinetic and affinity output of our ForteBio Octet BLI platform with the data analysis capabilities of ActivityBase XE enables us to perform “high value” screening within early antibody discovery.

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Technical Guide Biotinylation of Protein for Immobilization onto Streptavidin Biosensors

The interaction between streptavidin and biotin is widely used as a system for the rapid, stable and irreversible non-covalent binding of biological molecules. The Octet® platform’s Streptavidin biosensors have been developed for the immobilization of biotinylated ligands for both quantitation and kinetic applications. The first protein immobilized onto the Streptavidin biosensors must be biotinylated prior to assaying on the Octet system.

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Technical Guide Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors

ForteBio Streptavidin biosensors enable the immobilization of a biotinylated ligand onto the biosensor surface. The immobilized molecule can then be used in subsequent kinetic or custom quantitation applications.

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White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

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