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2-Channel Entry-Level Octet System: Octet K2

High performance, research-friendly kinetics characterization

Octet K2 System

The Octet® K2 2-channel System brings you unprecedented access to exquisite biomolecular interactions data. Priced for startup academic and biotech labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 System characterizes protein-protein and protein-small molecule binding interactions for early research and discovery. The multipurpose system also provides quantitative information by measuring active protein concentrations — even in complex mixtures like cell culture supernatants and lysates.

  •  

    Sensitivity for small molecule analysis

    The Octet K2 System enables the measurement of interactions between small molecules down to 150 Da in size, and their larger binding partners to determine accurate kinetics rates.

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    Flexible second channel for use as a reference or more samples

    Unique 2-channel design have dedicated spectrometers for simultaneous referencing or added capacity.

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    Simple setup for protein and antibody characterization

    Template driven interface allows new users to get started quickly for protein and antibody characterization.

  • Kinetic analysis

    The Octet K2 System monitors binding events in real time to calculate on rates (ka), off rates (kd), and dissociation constants (KD). The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference. Samples are loaded in standard microplates, eliminating fluidic line maintenance and expanding the number of samples that can be run in an assay. With predefined templates to follow in Octet data acquisition software, the system streamlines setup prior to running an assay and minimizes training needs. The software then gives users a range of parameters and metrics for analyzing acquired data in a powerful yet intuitive software interface.

  • Quantitative measurements

    Utilizing ForteBio's one-step Dip and Read™ assays, the Octet K2 System directly measures the presence of specific proteins and other molecules in solution. Accurate and reproducible concentrations can be determined in as little as two minutes per sample.

  • Biosensor Re-racking

    The Octet K2 System provides the ability to rerack biosensors at the end of an experiment. Biosensor reracking provides enhanced flexibility in loading ligands on biosensors and reduces operational costs when biosensors are loaded with precious target reagents.

large molecule

Large Molecule Characterization

With pre-defined templates to follow in Octet Data Acquisition software, the Octet K2 system streamlines setup prior to running an assay and minimizes training needs. Octet Data Analysis software then gives users a range of parameters and metrics for analyzing acquired data, in a powerful yet intuitive software interface

small molecule

Small Molecule Characterization

The Octet K2 system monitors binding events in real time to calculate on rates (ka), off rates (kd), and dissociation constants (KD). The system's two channels can be used to measure samples independently or in tandem, pairing the sample read with a dedicated reference.

 

2-Channel Entry-Level Octet System: Octet K2

Technology

Bio-Layer Interferometry (BLI) based on fiber optic biosensors

Sample volume

180–220 µL/well
Nondestructive testing, easily recovered

Data presentation

Sensorgrams displaying kinetic traces or concentration binding rates
Epitope binning and cross-blocking matrices and trace overlays
Tabulated kinetic or concentration data

Automation

Up to 2 assays in parallel

Orbital flow capacity

Static or 400–1500 rpm

Analysis temperature

Ambient + 4°C to 40°C, in 1°C increments

Association rate constant (ka)

101 to 107 M-1 S-1

Dissociation rate constant (kd)

10-6 to 10-1 s-1

Affinity constant (KD)

1 mM – 10 pM

Baseline noise (RMS)

<3.5 pm

Baseline drift

< 0.1 nm/hour

Quantitation range

• 0.05 μg/mL to 2000 μg/mL (Human IgG and Protein A Biosensor)
• Protein- and assay-dependent

Quantitation precision

CV <10%

Weight

58 lb (26.3 kg)

Dimensions (H x W x D)

18.6 in (H) x 17 in (D) x 20.8 in (W)
47 cm (H) x 43 cm (D) x 53 cm (W)

Sample type

Proteins, antibodies, peptides, serum containing media (up to 25%), DMSO containing buffers, virus-like particles, untreated cell culture supernatants and crude cell lysates

Sample plate

Standard, 96-well, black, flat-bottom microplate

Molecular weight detection

>150 Da

Resources of 2-Channel Entry-Level Octet System: Octet K2

Application Note A Fast and High Precision Influenza Vaccine Potency Assay

Vaccines are biological preparations that contain agents resembling disease causing microorganisms, and can improve immunity against a specific disease. They are typically prepared from inactivated or weakened forms of the microbe or its toxins, or surface proteins.

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Application Note Cross-competition or Epitope Binning Assays on the Octet HTX System

Epitope binning is a term used to describe segmentation of a panel of monoclonal antibodies (mAbs) into “bins” based upon the antigen region, or epitope, bound by each antibody.

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Application Note Generating Reliable Kinetic Data for Protein-Ligand Interactions

The Community Structure Activity Resource (CSAR, www.csardock. org) group is developing a database of high quality protein-ligand structures and the corresponding binding affinities. The data will be provided from in-house experiments and community collaborations. The proteins are generally well-studied structures that have been targeted in drug discovery projects.

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Application Note Validated Quantitation and Activity Assay of Antibody Fragment Molecule (Fab) for Process Development and Quality Control

The group was able to develop a working Fab activity assay on the Octet RED system in less than a week. Relative to the overnight incubation and four-hour assay time of their ELISA protocol, the Octet assay provided an analysis time of only one hour per 96-well microplate, including sample preparation time. This Octet assay was used to monitor Fab activity for all process development studies.

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Application Note Analysis of Fc-gamma Receptor-IgG Interactions on the Octet Platform

Fc gamma receptors (FcγRs) are membrane glycoproteins with affinity for the Fc region of immunoglobulin G (IgG). FcγRs expressed on the surface of immune effector cells play a key role in initiating Fc effector functions such as antibody-mediated cell-dependent cytotoxicity (ADCC)1, which is a major mechanism of action of therapeutic monoclonal antibodies

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Application Note Analysis of FcRn-Antibody Interactions on the Octet Platform

The Fc region of human IgG contributes to a number of beneficial biological and pharmacological characteristics of therapeutic antibodies.

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Application Note Customized quantitation of recombinant therapeutic proteins using High Precision Streptavidin biosensors (SAX)

The accurate determination of recombinant protein titer is critical to the selection of high-producing clones during cell line development and in optimization of bioreactor conditions during production of therapeutic proteins.

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Application Note Instant Determination of Protein Presence Using the BLItz System

The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a matter of seconds with high specificity and sensitivity, even in crude samples.

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Application Note CaptureSelect affinity ligands for antibody detection and characterization

BAC’s CaptureSelect affinity ligands provide a unique group of molecules that can be designed for almost any purification target, with as narrow or as broad specificity as is required. The fragments are derived from the unique heavy chain antibodies found in Camelidae. Heavy chain antibodies are devoid of the entire light chain and CH1 domain found in conventional antibodies (Figure 1). Without the light chain, antigens are bound only by the variable domain of the heavy chain (VHH), without a loss in binding affinities compared to conventional antibodies.

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Application Note Octet Potency Assay: Development, Qualification and Validation Strategies

Kinetic analysis of biomolecular interactions is critical during drug discovery and development. The affinity of an interaction directly affects the dose required for a biopharmaceutical to be effective.

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Application Note Rapid, reliable quantitation of Fc-Fusion protein in cell culture supernatants

The cell line development group at Biogen IDEC needed a robust assay for the measurement of Fc-fusion protein (Protein 1) in crude cell culture supernatants. The group had historically used HPLC for protein quantitation during screening and selection of promising mammalian clones at every scale-up step, from 96- well microplates to 3L-bioreactors.

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Application Note Converting an ELISA Assay into an Octet Quantitation Assay

Quantitation assays on the Octet platform have many similari- ties to enzyme-linked immunosorbent assays (ELISA). Both are performed on a solid support on which the capture molecule is im- mobilized and the analyte is bound from solution. Signal reported in the assay is either directly or inversely proportional to the amount of bound analyte. In fact, Octet quantitation assays can be considered automated forms of ELISA.

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Application Note Optimizing protein-protein and protein-small molecule kinetics assays

Kinetic analysis of protein-protein and protein-small molecule interactions is a key application for real-time, label-free systems such as the Octet® instrument family. The Octet platform is currently utilized in many segments of the pharmaceutical and biotherapeutic drug development processes such as early discovery, process development, late-stage clinical trials and manufacturing/QC.

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Application Note MAb Quantitation: Protein A HPLC vs. Protein A Bio-layer Interferometry

There are currently 30 monoclonal antibodies approved by the FDA as biotherapeutic agents, representing the most rapidly growing class of new drugs. Despite advances in downstream processing technology, affinity purification of monoclonal antibodies using Protein A chromatography is still the industry standard. In order to use Protein A resin as productively as possible it is important to load the resin at close to its dynamic binding capacity (DBC)

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Application Note Enhancing Efficiency and Economics in Process Development and Manufacturing of Biotherapeutics

Analytical techniques that measure protein quantity and quality are used in nearly all stages of research, process development and manufacturing of biotherapeutics. UV spectroscopy, ELISA and HPLC have been in use for decades for protein quantitation in physiologi- cal and process samples, and continue to be the workhorses despite their many limitations.

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Application Note Kinetic Analysis of Antibody Binding to an Expressed Membrane Protein on Captured Lipoparticles

Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular interactions critical to mapping the interactome as well as drug discovery efforts. Due to their integration into a lipid bilayer, in vitro characterization of molecular interactions of membrane proteins presents a unique challenge compared to soluble proteins.Membrane proteins govern the majority of input and output signals of cells and represent the largest class of pharmaceutical drug targets, making the analysis of their molecular interactions critical to mapping the interactome as well as drug discovery efforts. Due to their integration into a lipid bilayer, in vitro characterization of molecular interactions of membrane proteins presents a unique challenge compared to soluble proteins.

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Application Note Reducing Variability in Small Molecule Screening and Kinetics Applications

Minimizing the variability of background signals is a key parameter to the success of demanding applications such as small molecule analysis1. By reducing the background variability, smaller positive signals can successfully be resolved, providing an increase in assay sensitivity. This application note demonstrates the use of a reference biosensor with biotinylated streptavidin for increasing signalto-noise levels, thereby improving the detection of small molecules, including fragments.

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eBook Label-free detection: technologies, key considerations, and applications

Interactions between biomolecules serve as key triggers for many biological processes and, therefore, provide perfect targets for drug discoveries.

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Brochure Octet Systems

Your research should be guided by your imagination, not by the limitations of your label-free system. The Octet® platform’s flexible, open-format design and comprehensive menu of biosensors lets you attack research challenges in unique new ways. Perform truly comprehensive structure/function studies with large panels of structural variants.

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Data Sheet Anti-Penta-HIS (HIS1K) Biosensors

The polyhistidine tag, commonly known as His-tag, is fused to recombinant proteins as a means of facilitating detection and purification. The Dip and Read™ Anti-Penta-HIS (HIS1K) Biosensor consists of high affinity, high specificity Penta-His antibody from QIAGEN pre-immobilized on a ForteBio fiber optic biosensor.

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Data Sheet High Precision Streptavidin (SAX) Biosensors

Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors enable quick and easy modification and customization of the biosensor with any biotin-tagged molecule for quantitative and kinetic measurements.

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Data Sheet Anti-GST Biosensors

Glutathione-S-transferase (GST) is commonly fused to recombinant proteins to facilitate detection and purification, and increase solubility.

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Data Sheet Amine Reactive Second-Generation Biosensors

The Dip and Read™ Amine Reactive 2nd-Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes.

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Data Sheet Octet system qualification products and services

Regulations and global standards, including US FDA 21 CFR Part 11, EU GMP Annex 15 and USP <1058>, require documented verification that your instruments are delivered, installed and routinely calibrated and functioning according to their operational specifications. ForteBio offers a full line of qualification products and services to help you meet your initial and ongoing system qualification requirements.

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Data Sheet Anti-Mouse IgG Fc Capture (AMC) Biosensors

Dip and Read™ Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes.

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Data Sheet Octet K2 System

The Octet K2 2-channel system brings you unprecedented access to exquisite biomolecular interactions data. Priced for start-up labs, low volume users are no longer bound by the trade-off between cost and performance when choosing a label-free assay system. The Octet K2 system detects protein-protein and protein-small molecule interactions, with molecules down to 150 daltons in size.

Read more

Data Sheet Protein L Biosensors

Dip and Read™ Protein L (ProL) biosensors provide a rapid and direct method for quantifying a broad set of kappa light chain containing immunoglobulins, including whole molecules, FAb fragments and single chain variable fragments, in buffer, conditioned media or complex matrices.

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Data Sheet Protein G Biosensors

Protein G biosensors provide a rapid and direct method for quantification of many types of mammalian IgG from buffer, media or other complex matrices.

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Data Sheet Streptavidin (SA) Biosensors

ForteBio Streptavidin (SA) biosensors are designed for immobilization of biotin labeled proteins for use in assaying protein:protein interactions using the Octet® platform. The systems support applications for kinetics characterization and quantitation of analytes binding to the immobilized protein.

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Data Sheet Octet RED384 System

ForteBio’s Octet® RED384 system is designed for increased throughput for label-free protein quantitation and kinetic characterization. Get accurate concentration, kinetic constants, and affinity data for protein-protein, small molecule-protein and other fast-binding interactions – all with Dip and Read simplicity.

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Data Sheet Anti-human IgG (AHQ) Biosensors (Fc-specific)

ForteBio’s Dip and Read™ Anti-Human IgG (AHQ) biosensors, in conjunction with the Octet® platform, are designed for monitoring antibody concentrations from crude lysates and cell culture supernatants. Using Anti-Human IgG biosensors, the Octet platform supports applications from cell culture screening to purification monitoring during the process development and production of therapeutics.

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Data Sheet Octet Software Version 10.0: Data Acquisition, Data Analysis and Data Analysis High Throughput (HT)

Octet® software provides an intuitive and easy to use interface for data acquisition and analysis on all Octet instruments, enabling label-free kinetic, affinity, epitope binning, activity, concentration and screening applications.

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Data Sheet Octet software comparison

ForteBio Octet Data Acquisition and Data Analysis software is rapidly evolving to better address the growing applications and needs of our customers. These comparison tables provide a general guide to the features included in various versions of the software. The latest release, Version 11, is available for use on all Octet systems. For more information or for upgrade options, please contact your local sales representative or fill out our software download request form.

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Data Sheet Anti-human IgG Fc Capture (AHC) Biosensors

Capture-based immobilization of human Fc-containing proteins Regenerable and cost-effective format Compatible with crude samples and complex media

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Data Sheet Anti-Human Fab-CH1 2nd Generation Biosensors

Human antibodies are the most vital research candidates in drug discovery and development of bio-therapeutics. The detection and characterization of human IgG is of paramount importance for research scientists.

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Flyer BLI Biosensors Selection Guide

Read this document which is helpful in the selection of Biosensor with their detail information.

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Scientific Poster Detection of STEC Strains O157:H7, O26 and O111 using Biosensor System

Escherichia coli O157:H7, a strain of enterohemorrhagic, Shiga toxin-producing E. coli (STEC), causes gastroenteritis, resulting in (bloody) diarrhea and sometimes acute kidney failure due to HUS (hemolyticuremic syndrome).

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Scientific Poster Octet BLI systems – unmatched versatility for discovery, development and quality control

Traditional methods for analyzing the kinetics of biomolecular interactions such as ELISA are often cumbersome and may require rigorous sample preparation. In addition, ELISA may not provide the information required for the confident understanding of a drug molecule’s MOA. In contrast the 96-well sample plate based Octet® family of instruments (Figure 1) such as the Octet RED96e system provide researchers with an easy to use real time label free system that can detect the interactions of a diverse range of biomolecules; from small molecules to proteins to mammalian cells. The Octet platform offers an advanced fluidics-free approach with a wide variety of off-the-shelf Dip and Read™ biosensors for rapid binding kinetics and quantitation analysis, enabling direct detections of not only purified biomolecules, but even those in complex media such as cell culture supernatants and lysates. The 96-channel Octet HTX system performs quantitation of 96 samples in as little as 2 minutes, and kinetic screening of 384 samples in 15 minutes. Analysis can be done using a single channel or up to 96 channels, enabling more flexibility in sample throughput when needs change. In addition, the Octet systems have been developed to operate reliably in a regulated environment. Fortebio offers 21 CFR Part 11 software and a full line of GxP products and services as part of the GxP Package. To demonstrate the performance of the BLI technology, applications in protein-protein and protein-small molecules interactions will be described.

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Technical Guide Biotinylation of Protein for Immobilization onto Streptavidin Biosensors

The interaction between streptavidin and biotin is widely used as a system for the rapid, stable and irreversible non-covalent binding of biological molecules. The Octet® platform’s Streptavidin biosensors have been developed for the immobilization of biotinylated ligands for both quantitation and kinetic applications. The first protein immobilized onto the Streptavidin biosensors must be biotinylated prior to assaying on the Octet system.

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Technical Guide Dip and Read™ Amine Reactive Second-Generation (AR2G) Reagent Kit

The Amine Reactive 2nd Generation (AR2G) Reagent Kit is intended for use with the AR2G biosensor to enable covalent immobilization of a protein, peptide or other primary amine containing biomolecule onto the AR2G biosensor surface via a stable amide bond. Immobilization of proteins is achieved through standard EDC-catalyzed amide bond formation to create a covalent bond between a reactive amine on the protein and the carboxy-terminated biosensor surface. The kit contains sufficient reagents to perform 1000 standard immobilization reactions.

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Technical Guide CHO Host Cell Protein Detection

Host cell proteins (HCPs) are contaminants found in biopharmaceuticals expressed in bacterial, yeast or mammalian production cell lines. Among protein expression cell lines, Chinese hamster ovary (CHO) cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics

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Technical Guide Dip and Read™ Amine Reactive Second-Generation (AR2G) Biosensors

Amine Reactive 2nd Generation (AR2G) biosensors enable kinetic characterization of macromolecular interactions between purified proteins and target analytes.

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Technical Guide High Precision Streptavidin Biosensor (SAX) Quantitation and Kinetic Assays

Streptavidin-coated surfaces are widely used as a simple and straightforward method of molecular immobilization. Utilized with Bio-Layer Interferometry (BLI), Streptavidin biosensors enable quick and easy modification and customization of the biosensor with any biotintagged molecule for quantitative and kinetic measurements.

Read more

Technical Guide Dip and Read™ Anti-Mouse IgG Fc Capture Biosensors

Anti-Mouse IgG Fc Capture (AMC) biosensors enable kinetic characterization of macromolecular interactions between mouse Fc-containing proteins and target analytes (Figure 1). Immobilization of mouse Fc-containing proteins is achieved through a factory immobilized anti-mouse Fc-specific antibody whose high affinity for the mouse Fc domain provides the stable baseline required for demanding kinetics applications. Cost-effective regeneration of the biosensors and the ability to directly immobilize mouse Fc-containing proteins from crude matrices make AMC biosensors extremely useful in high-throughput applications.

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Technical Guide Host Cell Protein Detection

Host cell proteins (HCPs) are contaminants found in biopharmaceuticals that originate from the production cell lines of bacterial, yeast or mammalian cell culture. HCPs can reduce drug efficacy and delay or kill promising drug candidates through adverse patient reactions.

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Technical Guide Regeneration Strategies for Streptavidin Biosensors on the Octet Platform

The ForteBio Octet platform offers powerful tools for the real-time, label-free analysis of protein-protein interactions. Together, the Octet systems and Streptavidin (SA) biosensors provide a flexible format for kinetic analysis and kinetic screening for any biological interaction in which one molecule can be biotinylated. Read more

Technical Guide Batch Immobilization of a Biotinylated Ligand onto Streptavidin Biosensors

ForteBio Streptavidin biosensors enable the immobilization of a biotinylated ligand onto the biosensor surface. The immobilized molecule can then be used in subsequent kinetic or custom quantitation applications.

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Technical Guide Anti-Penta-HIS (HIS1K) Biosensors for Label-free Analysis of His-tagged Proteins

The polyhistidine-tag (His-tag) is a common peptide tag added to recombinant proteins during cloning. Many tools have been developed that enable this tag to be used for detection and purification of tagged proteins. Read more

Technical Guide Rapid Analysis of Fab Fragments and IgG with Anti-Human Fab-CH1 2nd Generation (FAB2G) Biosensors

Anti-Human Fab-CH1 2nd Generation (FAB2G) biosensors from Pall ForteBio come pre-immobilized with a high affinity ligand that is specific for the CH1 region of Human IgG. \

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Technical Guide Anti-HIS (HIS2) Biosensor Quantitation Assays

The polyhistidine-tag (HIS-tag) is a common peptide tag fused to recombinant proteins during cloning. Many tools have been developed that enable this tag to be used for detection and purification of tagged proteins. The Anti-HIS (HIS2) Biosensor provides a rapid, label-free method for quantitation of HIStagged proteins on Octet® and BLItz® systems. This biosensor comes pre-immobilized with the next-generation high-affinity, high-specificity anti-HIS antibody from MBS (Maine Biotechnology Services), and is ready to use for detection and quantitation of HIS-tagged proteins.

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Technical Guide Ni-NTA Biosensor Quantitation Assays

A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purification.

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Technical Guide Octet kinetics assay: method development guideline

The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. The procedures described are for developing binding kinetics assays for release testing of product batches and related applications. The document is intended as a general guideline only. Actual method development procedures may be different as they may be product dependent.

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Technical Guide Ni-NTA Biosensor Kinetic Assays

A polyhistidine tag (also known as hexa histidine-tag, 6xHIS-tag, or by the trademarked name HIS-tag) is commonly fused to recombinant proteins to facilitate their detection and purification. 

Read more

Technical Guide Anti-GST Biosensor Quantitation Assays

Glutathione-S-transferase (GST) is commonly fused to recombinant proteins as a means of facilitating detection, purification and increasing solubility.

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Technical Guide GST Biosensor Kinetics Assays

Glutathione-S-transferase (GST) is commonly fused to recombinant proteins to facilitate detection and purification, and to increase solubility.

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White Paper Octet platform: a complete solution for lot release and in-process testing of biologics in GxP laboratories

Bio-Layer Interferometry (BLI) is an optical analytical technique that analyzes the interference pattern of white light reflected from two surfaces: a layer of immobilized protein on the biosensor tip and an internal reference layer (Figure 1A). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real time (Figure 1A and 1B).

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White Paper Writing an Equipment Grant Proposal for Biomolecular Interactions Research: Bio-Layer Interferometry (BLI) and Octet Systems

In this white paper, guidelines for preparing an instrumentation grant application will are provided for principal investigators interested in acquiring an Octet® system, the label-free BLI biosensor platform for biomolecular interaction analysis.

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Application Overview Characterizing Membrane Protein Interactions by Bio-Layer Interferometry (BLI)

The cell membrane separates the intracellular components from the extracellular environment. Cell membranes consist of various components such as phospholipids, glycolipids, and cholesterol, in combination with integral and peripheral proteins.

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