Personal assay BLItz System

Label-free assays in a drop

Drop. Read. Done.

The BLItz® System brings label-free analysis to individual researchers, and is affordably priced so everyone can have one. With this personal assay system, you can run protein quantitation and kinetics experiments in only 4 µL of sample—right at your own lab bench.

Label-Free Analysis on BLItz SystemLabel-Free Analysis on BLItz System
  •  

    Simple and rapid

    The BLItz System enables the detection of specific mAbs, even in unpurified samples, in as little as 30 seconds.

  •  

    Save sample

    Affinity, concentration, and binding kinetics can be measured right at the bench in a 4 µL drop of sample.

  • Instant protein presence

    Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz System complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.

  • Faster protein quantitation

    Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz System gives you accurate quantitation in seconds compared to traditional methods that can often take hours.

  • Easy reagent QC testing

    Rapidly identifying specific proteins with immunoassay-based techniques like ELISA or HPLC add many hours to the process and delay results. With the BLItz System, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples.

  • Effortless kinetics

    Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4µL of sample. With both a small price and a small size, the BLItz System can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.

  • Smarter assay development

    Monitoring the effects of changing conditions on binding interactions in real time, the BLItz System lets you develop better immunoassays in only a few minutes. Find the best antibody pairs, identify the best assay parameters, and even build and optimize an ELISA step by step.

Kinetics

Easily Determine Interactions Kinetics

Get rate and affinity constants for binding interactions (ka, kd, KD) in minutes using only 4 µL of sample. With both a small price and a small size, the BLItz system can be your own personal label-free kinetics system. Ready to go right out of the box, its simple workflows let you run assays right at your lab bench with ease.

Protein Presence Instantly

Instantly Detect Presence of Specific Protein

Detect the presence or absence of active proteins in just a drop of sample, and easily identify specific proteins in complex solutions. Data from the BLItz system complements existing methods like blots and gels, and its real-time binding curves give instant visual confirmation.

Rapidly Quantify your Protein with Confidence

Rapidly Quantify your Protein with Confidence

Measure protein concentration without labeling or secondary reagents — even in crude media. With a wide dynamic range and sensitivity down to a few ng/mL, the BLItz system gives you accurate quantitation in seconds compared to traditional methods that can often take hours.

 

Personal assay BLItz System

Power

100–240 V AC, 0.2–0.1 A, 50/60 Hz, 8 W (18 W peak)

Technology

Bio-layer Interferometry (BLI)

Sample volume

4 µL

Weight

7.2 lb (3.3 kg)

Dimensions

6.8” H x 6.0” W x 8.7” D (17.4 cm H x 15.3 cm W x 22.2 cm D)

Resources of Personal assay BLItz System

Application Note Fast Quantitation of Proteins and Antibodies Using the BLItz System

Traditional techniques for determining concentration of a target protein such as ELISA and HPLC are both elaborate and timeconsuming, especially when analyzing complex matrices. For bioprocess development and production applications, the use of more rapid protein analysis techniques enables timely, informed process decisions.

Read more

Application Note Instant Determination of Protein Presence Using the BLItz System

The BLItz system provides a simple, rapid Dip and Read™ approach to protein analysis in an affordably priced personal assay system. Protein and antibody detection can be performed in a matter of seconds with high specificity and sensitivity, even in crude samples.

Read more

Application Note Best practices for performing quantitation assays using the BLItz System

The BLItz® system provides a simple, rapid Dip and Read™ approach for protein quantitation in a small, affordably priced personal assay system. With BLItz, proteins and antibodies can be quantitated in a matter of seconds with high specificity and sensitivity, even in crude samples. The system utilizes the same proprietary Bio-Layer Interferometry (BLI) technology as ForteBio’s Octet platform, enabling real-time analysis of interactions on the surface of disposable fiber optic biosensors.

Read more

Brochure BLItz Label-free Assay in a Drop

The BLItz® system finally makes label- free analysis available to individual researchers. Priced so everyone can have one, you can now monitor protein binding interactions, quantify protein expression, analyze column fractions, QC reagents, and run protein engineering studies right at your own bench.

Read more

Data Sheet Qualification and Compliance Tools for the BLItz® System

Affordably priced and easy to use, the BLItz system is enabling labelfree detection and analysis across protein manufacturing, purification, and processing workflows.

Read more

Newsletter NEW Protein G Biosensor Available for the Octet Platform!

Immunoglobulin-binding proteins such as Protein A, Protein G and Protein L are commonly used to purify, immobilize or detect immunoglobulins. They have different binding profiles with respect to species, antibody type, and the portion of the antibody that is bound. Protein A biosensors are currently used for human and other IgG quantitation on the Octet platform in a variety of applications including cell line development, clone selection, process optimization and production monitoring.

Read more

Scientific Poster Label-free Analysis from Microliter Amounts of Sample

Label-free Analysis from Microliter Amounts of Sample

Read more

Technical Guide Anti-Human Fab-CH1 (FAB) Biosensor Quantitation Assays

The Anti-Human Fab-CH1 Biosensor consists of a high affinity antihuman CH1 affinity ligand pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the AntiHuman Fab-CH1 Biosensor provides a rapid and label-free method for Human Fab/F(ab’)2/IgG quantitation and kinetic analysis. The high specificity of the antibody fragment-based biosensor enables direct analysis of human Fab/F(ab’)2/IgG analytes in crude lysates, column eluents, cell lysates and cell culture supernatants, serving as a time-saving alternative to traditional analytical methods. For more information on kinetic analyses using the Anti-Human FabCH1 Biosensor, please see ForteBio Technical Note 34, Anti-Human Fab-CH1 Biosensor Kinetics Assays.

Read more

Technical Guide Anti-Human Fab-CH1 Biosensor Kinetics Assays

The Anti-Human Fab-CH1 Biosensor consists of a high-specificity anti-Human Fab-CH1 antibody fragment pre-immobilized on a ForteBio biosensor. In conjunction with the Octet® or BLItz™ systems, the Anti-Human Fab-CH1 Biosensor provides a rapid and label-free method for Human F(ab’)2/Fab/IgG quantitation and kinetic analysis. The high specificity of the antibody fragment-based biosensor enables direct analysis of Human F(ab’)2/Fab/IgG analytes in crude lysates, column eluents, cell lysates and cell culture supernatants, serving as a time-saving alternative to traditional analytical methods. For more information on quantitation analyses using the AntiHuman Fab-CH1 Biosensor, please see ForteBio Technical Note 33, Anti-Human Fab-CH1 Biosensor Quantitation Assays.

Read more

Technical Guide Enabling Depth Filter Selection with Rapid Detection of Monoclonal Antibodies in Culture Using the BLItz System

Harvesting and recovering monoclonal antibodies (mAb) from mammalian cell culture bioreactor samples is just the first step of a closely coordinated chain of purification and processing steps. The ability to provide quick checks at-line for titer provides valuable insight into process variability and opportunities for increased con- trol over them.

Read more

Technical Guide BLI selection guide

BLI selection guide

Read more

Technical Guide Octet kinetics assay: method development guideline

The purpose of this document is to describe the procedure for developing a ligand binding kinetics assay on Octet® QKe, RED96, RED96e, RED384 and HTX instruments. The procedures described are for developing binding kinetics assays for release testing of product batches and related applications. The document is intended as a general guideline only. Actual method development procedures may be different as they may be product dependent.

Read more

Technical Guide Biotinylate antibody from stock which contains carrier protein

Biotinylate antibody from stock which contains carrier protein (Recommended procedure for use with FortéBio streptavidin biosensors)

Read more

Tutorials & Videos Flexible Monitoring of Small Molecules with Aptamer Affinity Reagents Using Bio-Layer Interferometry

Tutorials & Videos Kinetics on the Octet Systems What Lies Beneath the Curves

Tutorials & Videos Protein Concentration Measurements in Crude Samples in a Dip and Read Format

Tutorials & Videos Using the BLItz Personal Assay System to Overcome Bioprocess Development Bottleneck

Product Part Number
BLItz® System Drop Holder, 6 Pack 45-5004
BLItz® System Lamp Change Kit 45-5002
Depot Service Agreement BLItz® System 98-0007
IQOQ Validation Manual and Testing Kit for BLItz® System 41-0162

We use cookies to personalize content and improve your browsing experience.

We also use analytics & advertising services. To opt-out click for more information.