Anti-Idiotypic Nanobody as Citrinin Mimotope from a Naive Alpaca Heavy Chain Single Domain Antibody Library

Xu Y, et al., 407(18):5333-41, Anal Bioanal Chem, 2015

The article describes development of a strategy to obtain β-AI VHH as a CIT mimotope using a phage display VHH library. The authors claim several advantages of this strategy as compared to conventional AId development methods. A Pall ForteBio BLItz system was used to estimate binding kinetics of VHH or CIT-OVA to McAb. The Ni-NTA and AR2G biosensors were used to capture HIS-tagged VHH and CIT-OVA, respectively.

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