Blocking Interaction of Viral gp120 and CD4-expressing T Cells by Single-stranded DNA Aptamers

Zhao N, et al., 51C:10-18, Int J Biochem Cell Biol, 2014

The authors generated and screened single stranded DNA (SSDNA) aptamers using a systematic evolution of ligands by exponential enrichment (SELEX) approach and next generation sequencing. They further demonstrated that the SSDNA aptamers selectively bound CD4 on human cells and disrupted the interaction of viral gp120 to CD4 receptors, which is one of the prerequisite steps in HIV-1 infection and transmission. Binding characterization was performed on the Blitz system using Anti-His biosensors.

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