O, 2 (12) 1227-1243, Oncotarget, 2011
This publication describes the development of high-affinity mAbs to the human folate receptor alpha (FRA). Epitope binning studies on the Octet QK system were performed to identify mAbs that cross-compete for FRA binding. Biotinylated rFRA was loaded onto Streptavidin biosensors, followed by incubation with a primary saturating anti-FRA mAb. The biosensors then were moved to wells containing secondary competing anti-FRA mAbs.