Secretion of the Housekeeping Protein Glyceraldehyde-3-phosphate Dehydrogenase by the LEE-encoded Type III Secretion System in Enteropathogenic Escherichia coli

Aguilera, L., et al., 44(6), 955-962, Int J Biochem Cell Biol, 2012

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional housekeeping protein secreted by pathogens and involved in adhesion and/or virulence. The authors demonstrate a novel interaction between GAPDH and the chaperone CesT by means of pull down experiments, overlay immunoblotting and BLI . The Octet RED system with Amine Reactive biosensors was used to study the GAPDH-Cest interaction. Biosensors with immobilized CesT were exposed to various concentrations of purified GAPDH (0-5.5 micromolar).

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