Strand-specific Recognition of DNA Damages by XPD Provides Insights into Nucleotide Excision Repair Substrate Versatility

Buechner C, et al., 289(6):3613-24, J Biol Chem , 2014

The article looked into the mechanisms by which the helicase XPD (xeroderma pigmentosum group D) protein accomplish broad substrate adaptability during nucleotide excision repair (NER). Timely recognition and repair of DNA damages caused by UV radiation or chemical substances is crucial for cell viability. The authors used biophysical methods such as single molecule analysis by atomic force microscopy and BLI to investigate different recognition strategies of XPD for different types of damage. The BLI estimates on an Octet RED system allowed understanding of the affinities between immobilized DNA (single stranded or double stranded oligonucleotides containing different motifs or chemical alterations) and serial dilutions of XPD. The DNA ligands (labeled with biotin) immobilized onto SA biosensors.

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