SPR Biosensor Surface Chemistries for Pioneer Systems

We offer a selection of surface chemistries for use on the Pioneer and Pioneer FE platforms — from amine reactive surfaces with varying capacities to pre-immobilized capture chemistries. Our high quality SPR biosensors enable researchers to optimize for a diverse array of ligand types and applications, ensuring generation of high quality data from interaction analysis studies.

SPR Biosensor Selection Guide

Biosensor Description
CDL (Replaces COOH2) The CDL biosensor is a medium capacity three-dimensional chemistry that comprises a thin Dextran layer with carboxylic acids. The Dextran spacer is excellent for reducing non-specific binding and is biocompatible with a wide range of molecules. Amine coupling is the standard immobilization method for CDL and its coupling capacity is between the COOH1 and CDH biosensors. Typical uses are measuring kinetics of intermediate (>1 kDa) to large molecules (>25 kDa).
CDH (Replaces COOHV) This CDH biosensor is a high capacity three-dimensional chemistry that uses a medium length Dextran hydrogel spacer with carboxylic acids. This biosensor contains a high number of carboxylic acid functional groups that allows a large amount of target to be coupled onto the surface. This is particularly useful to observe the interactions of small molecules or for target that exhibit low binding activity. Amine coupling is the standard immobilization method for CDH. Its coupling capacity is the higher than the CDL biosensors.
PCH (Replaces COOH5) The PCH biosensor is a high capacity three-dimensional chemistry comprised of a linear polycarboxylate hydrogel layer. This biosensor contains a high number of carboxylic acid functional groups that allows a large amount of target to be coupled onto the surface. This enables analysis of interactions of fragments and small molecules or for targets that exhibit low binding activity. Amine coupling is the standard immobilization method for PCH and its coupling capacity is the highest of the carboxyl biosensors. This surface is also useful where a non-Dextran based hydrogel is desired.
SADH (Replaces BioCap) The SADH biosensor consists of Strepavidin immobilized in a three-dimensional carboxymethyl dextran hydrogel to allow for the selective irreversible capture of biotin containing ligands. It has moderate to high capacity making it ideal for analysis of intermediate (>1 kDa) to large (>25 kDa) molecule kinetics.
HisCap The HisCap biosensor is based on a three-dimensional non-dextran hydrogel chemistry. It is modified with Nitrilotriacetic acid (NTA) functional groups that chelate Nickel ions for the selective capture of 6X and 10X His-tagged targets. This surface can be partially regenerated by injecting Imidazole, EDTA or other similar reagents that compete for Nickel. Typical uses are small molecule or peptide kinetics with His-tagged protein targets.
COOH1 The COOH1 biosensor is a low capacity two-dimensional surface chemistry that comprises a polyethylene glycol (PEG) spacer terminated with carboxylic acids. This surface is particularly useful where lectin binding may occur or where Dextran coatings are not desired. Amine coupling is the standard immobilization method for COOH1 and its coupling capacity is the lowest of the carboxyl biosensors. Typical uses are large molecule (>25 kDa) kinetic assays.